 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on Sep 26, 2019 |
| Title |
The zingerone analog, acetyl zingerone, bolsters matrisome synthesis, inhibits MMPs, and represses IL-17A target gene expression [Experiment 2] |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
Zingerone (Z) is a phenolic alkanone derived from natural sources with anti-inflammatory and antioxidant effects. Acetyl zingerone (AZ) is a newly designed molecule that shares structural features with Z but is expected to have improved stability and antioxidant function. This study utilized microarrays to compare effects of Z and AZ on gene expression in reconstituted human epidermis. Both Z and AZ increased Notch pathway gene expression (NOTCH1, MAML3) and decreased expression of genes linked to extracellular matrix disassembly (MMP3, CTSV) and reactive oxygen species metabolism (PMAIP1, ARG2). Although Z and AZ each inhibited in vitro MMP-1, MMP-3 and MMP-12 activity, inhibition of MMP-3 and MMP-12 was greater with AZ. Moreover, AZ led to more consistent increases in the expression of genes encoding collagens (COL11A2), proteoglycans (VCAN) and ECM glycoproteins (SPARC). Finally, AZ opposed gene expression patterns associated with fibroblast senescence, keratinocyte differentiation, and IL-17A stimulation. These effects were AZ-specific and not replicated by Z. These results show that AZ improves extracellular matrix integrity with retinoid-like effects on differentiation and inflammation. Our findings provide rationale for clinical studies to understand benefits of AZ in the treatment or prevention of skin aging, or potentially, as a treatment for other human skin diseases.
Microarray analyses was performed to characterize global gene expression responses to acetyl zingerone (AZ) (50 μg/ml) in EpiDerm FT (RHE) tissue cultures.
|
| |
|
| Overall design |
EpiDerm FT (RHE) tissues were treated with CTL or AZ for a 24 hour period. Water was used as the control and the AZ concentration was 50 μg/ml. RNA was then extracted and samples were then sent for DNA microarray analysis (Agilent microarrays, Human version 8X60K).
|
| |
|
| Contributor(s) |
Swindell WR, Bojanowski K, Chaudhuri RK |
| Citation(s) |
31465741 |
| Submission date |
Jun 26, 2019 |
| Last update date |
Dec 28, 2019 |
| Contact name |
William R Swindell |
| E-mail(s) |
wswindel@umich.edu
|
| Organization name |
University of Michigan
|
| Department |
Dermatology
|
| Street address |
1500 E. Medical Center Drive
|
| City |
Ann Arbor |
| State/province |
MI |
| ZIP/Postal code |
48104 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL17077 |
Agilent-039494 SurePrint G3 Human GE v2 8x60K Microarray 039381 (Probe Name version) |
|
| Samples (4)
|
|
| This SubSeries is part of SuperSeries: |
| GSE133340 |
The zingerone analog, acetyl zingerone, bolsters matrisome synthesis, inhibits MMPs, and represses IL-17A target gene expression |
|
| Relations |
| BioProject |
PRJNA551212 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE133339_RAW.tar |
12.5 Mb |
(http)(custom) |
TAR (of TXT) |
| Processed data included within Sample table |
|
|
|
|
 |
Less...
More...