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| Status |
Public on Feb 16, 2020 |
| Title |
Deciphering human macrophage development at single-cell resolution |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Macrophages are the earliest emerging cells of the nascent immune system during embryonic development, and as innate immune cells constitutes an important first-line barrier against foreign organisms and pathogens. Rodent macrophages have been shown to infiltrate multiple organs at an early stage, developing symbiotically alongside these organs becoming tissue-resident macrophages (TRM) supporting tissue development and homeostasis. However, knowledge of the development and specialization of macrophages in the early human embryo is still limited. In order to study the spatiotemporal distribution and dynamic process of macrophage development in the early human embryo, we applied single-cell transcriptomic sequencing to generate a map of all CD45+CD235a- hematopoietic cells in human embryos of successive developmental stages from Carnegie stage (CS) 11 to 23. Here, we unravelled for the first time a map of macrophage heterogeneity across multiple anatomical sites and identified multiple macrophage subsets in the early human embryo, including various types of embryonic TRM (head, liver, lung and skin), and traced their developmental trajectories from yolk sac/embryonic liver-derived macrophages to their TRM specification in the head and liver based on core transcriptional factors. Altogether, our analyses provide a comprehensive characterization of the spatial and temporal dynamics of macrophage development in the early human embryo, and provides a reference for future study into human TRM function and embryonic development.
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| Overall design |
We sorted CD45+CD235a- hematopoietic cells using flow cytometry from human embryos at multiple Carnegie stages (CS11, CS12, CS13, CS15, CS17, CS20 and CS23) and sites (yolk sac, head, liver, blood, lung and skin), and then performed a modified single cell tagged reverse transcription (STRT) protocol.
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| Contributor(s) |
Bian Z, Huang T, Gong Y, Lee CZ, Shi H |
| Citation(s) |
32499656 |
| Submission date |
Jun 26, 2019 |
| Last update date |
Jun 28, 2020 |
| Contact name |
Tao Huang |
| E-mail(s) |
huang_tao945@126.com
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| Organization name |
The Fifth Medical Center of Chinese PLA General Hospital
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| Street address |
Dong Da Jie No.8
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| City |
Beijing |
| State/province |
Beijing |
| ZIP/Postal code |
100071 |
| Country |
China |
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| Platforms (1) |
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| Samples (9)
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| Relations |
| BioProject |
PRJNA551221 |
| SRA |
SRP212115 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE133345_Annotations_of_all_1231_embryonic_cells_updated_0620.txt.gz |
30.1 Kb |
(ftp)(http) |
TXT |
| GSE133345_Quality_controled_UMI_data_of_all_1231_embryonic_cells.txt.gz |
8.5 Mb |
(ftp)(http) |
TXT |
| GSE133345_RAW.tar |
9.7 Mb |
(http)(custom) |
TAR (of TXT) |
| GSE133345_all_1231_embryonic_cells_barcode.txt.gz |
5.5 Kb |
(ftp)(http) |
TXT |
| GSE133345_child_foreskin_barcode.txt.gz |
447 b |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
| Processed data are available on Series record |
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