|
| Status |
Public on Oct 01, 2019 |
| Title |
RNase H dependent Antisense oligonucleotides in the nucleus causes transcription termination |
| Organism |
Mus musculus |
| Experiment type |
Other
|
| Summary |
The aim of this study is to characterize Rnase H ASO mediated transcription events on chromatin.
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| |
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| Overall design |
chromatin associated RNA sequencing (chrRNA-seq): chromatin fraction were isolated from MHT cells after 24 hrs ASO free uptake. 14 different gapmer ASOs treatments targeting on 2 genes and 6 different controls were conducted for sequencing.
Precision nuclear run-on sequencing (PRO-seq): biotin labelled substrates were applied after ASO treatments. 6 different ASO treatments and 3 controls were applied for PRO-seq.
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| |
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| Contributor(s) |
Lai F, Damle S |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Jun 26, 2019 |
| Last update date |
Oct 02, 2019 |
| Contact name |
Sagar S Damle |
| E-mail(s) |
sdamle@ionisph.com
|
| Organization name |
Ionis Pharmaceuticals
|
| Department |
Functional Genomics
|
| Street address |
2855 Gazelle Ct
|
| City |
Carlsbad |
| State/province |
CA |
| ZIP/Postal code |
92010 |
| Country |
USA |
| |
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| Platforms (1) |
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| Samples (29)
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| Relations |
| BioProject |
PRJNA551276 |
| SRA |
SRP212127 |
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