|
| Status |
Public on Feb 26, 2020 |
| Title |
Single-cell RNA-seq analysis identifies meniscus progenitors and reveals the progression of meniscus degeneration |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
We identified seven clusters in healthy human meniscus, including five empirically-defined populations and two novel populations. Pseudotime analysis showed EC and FCP existed at the pseudospace trajectory start. MCAM (CD146) was highly expressed in two clusters. CD146+ meniscus cells differentiated into osteoblasts and adipocytes and formed colonies. We identified changes in the proportions of degenerated meniscus cell clusters and found a cluster specific to degenerative meniscus with progenitor cell characteristics. The reconstruction of four progenitor cell clusters indicated that FCP differentiation into DegP was an aberrant process. Interleukin-1β stimulation in healthy human meniscus cells decreased CD146+ cells and increased CD318+ cells, while TGFβ1 attenuated the increase in CD318+ cells in degenerated meniscus cells.
|
| |
|
| Overall design |
scRNA-seq was used to identify cell subsets and their gene signatures in healthy human and degenerated meniscus cells
|
| |
|
| Contributor(s) |
Sun H, Zhang Z |
| Citation(s) |
31871141 |
| Submission date |
Jun 27, 2019 |
| Last update date |
Feb 26, 2020 |
| Contact name |
Hao Sun |
| E-mail(s) |
sunhaojnsysu@foxmail.com
|
| Phone |
15017541945
|
| Organization name |
First Affiliated Hospital of Sun Yat-sen University,
|
| Department |
Joint surgery
|
| Street address |
Zhongshan 2nd Road
|
| City |
Guangzhou |
| State/province |
Guangdong |
| ZIP/Postal code |
510220 |
| Country |
China |
| |
|
| Platforms (1) |
|
| Samples (2) |
|
| Relations |
| BioProject |
PRJNA551470 |
| SRA |
SRP212209 |
