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Status |
Public on Jul 02, 2019 |
Title |
Transcriptomics analysis of gene expression in wildtype and NSUN2 knockdown T24 cell |
Organism |
Homo sapiens |
Experiment type |
Expression profiling by high throughput sequencing
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Summary |
Total RNA was isolated using TRIzol® Reagent (Ambion). The RNA concentration and quality were determined with NanoDrop, Qubit and agarose gel analysis. The Dynabeads® mRNA Purification Kit (Ambion) was used to enrich the mRNAs, which were used as templates for RNA-Seq library construction. RNA-Seq libraries was directly constructed by using KAPA mRNA Stranded mRNA-Seq Kit (KAPA) according to the manufacturer’s instructions. The libraries were sequenced on the Illumina HiSeq X-Ten platform at Novogene (Tianjin, CA) with paired-end 150 bp read length. The different genes expression between the control and NSUN2 knockdown cell were detected.
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Overall design |
Examination of gene expressive levels in wildtype and NSUN2 knockdown T24 cell
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Contributor(s) |
Chen X, Li A, Sun B, Xie D, Yang Y |
Citation(s) |
31358969 |
Submission date |
Jul 01, 2019 |
Last update date |
Apr 23, 2020 |
Contact name |
Bao-Fa Sun |
E-mail(s) |
sunbf@big.ac.cn
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Organization name |
Beijing Institute of Genomics (BIG) of Chinese Academy of Sciences (CAS)
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Street address |
Da-Tun Road
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City |
Beijing |
ZIP/Postal code |
100101 |
Country |
China |
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Platforms (1) |
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Samples (4)
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This SubSeries is part of SuperSeries: |
GSE133671 |
m5C Promotes Pathogenesis of the Bladder Cancer Through Stabilizing mRNAs |
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Relations |
BioProject |
PRJNA552053 |
SRA |
SRP212699 |