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| Status |
Public on Jul 02, 2019 |
| Title |
RNA m5C analysis in wildtype and NSUN2 knockdown T24 cell |
| Organism |
Homo sapiens |
| Experiment type |
Methylation profiling by high throughput sequencing
|
| Summary |
RNA fragmentation and bisulfite conversion were performed. In brief, 200 ng of mRNAs along with 1 ng in vitro transcribed mouse dihydrofolate reductase (Dhfr) spike-in RNA were used. The bisulfite treated samples were desalted with Nanoseq with 3K omega 500/pk centrifugal devices (PALL). Sequencing was performed on an Illumina HiSeq X-Ten sequencing system. The m5C sites were called using meRanCall from meRanTK (FDR < 0.01). The differential m5C sites were detected
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| |
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| Overall design |
Examination of m5C changes in NSUN2 knockdown samples
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| |
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| Contributor(s) |
Chen X, Li A, Sun B, Xie D, Yang Y |
| Citation(s) |
31358969 |
| Submission date |
Jul 01, 2019 |
| Last update date |
Apr 23, 2020 |
| Contact name |
Bao-Fa Sun |
| E-mail(s) |
sunbf@big.ac.cn
|
| Organization name |
Beijing Institute of Genomics (BIG) of Chinese Academy of Sciences (CAS)
|
| Street address |
Da-Tun Road
|
| City |
Beijing |
| ZIP/Postal code |
100101 |
| Country |
China |
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| Platforms (1) |
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| Samples (2) |
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| This SubSeries is part of SuperSeries: |
| GSE133671 |
m5C Promotes Pathogenesis of the Bladder Cancer Through Stabilizing mRNAs |
|
| Relations |
| BioProject |
PRJNA552056 |
| SRA |
SRP212701 |
