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| Status |
Public on Jul 02, 2019 |
| Title |
Gene expression data from IMR90 control, IMR90 shRRM2 and shRRM2/shp16 |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Transformed and tumorigenic cells require increased deoxyribonucleotide synthesis to fuel the genome replication that sustains their unregulated cell cycle and proliferation. Therefore, it is likely that the cell cycle and nucleotide metabolism are linked. The cell cycle inhibitor p16 is a critical tumor suppressor that is lost as an early event in many human cancers. While loss of p16 is known to play a role in deregulating the cell cycle, whether loss of p16 expression affects nucleotide metabolism is unknown.
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| Overall design |
mRNA profiles of IMR90 control, dNTP depletion-induced senesnce (shRRM2) and dNTP depletion-induced senescence bypass (shRRM2/shp16) were generated by deep sequencing, in triplicate, using HiSeq 2500 sequencer (Illumina)
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| Contributor(s) |
Buj R, Chen C, Dahl ES, Leon KE, Kuskovsky R, Maglakelidze N, Kutzler L, Lacko H, Lu Y, Gordan MB, Gowda R, Robertson GP, Warrick JI, Herlyn M, Imamura Y, Kimball SR, DeGraff DJ, Snyder NW, Aird KM |
| Citation(s) |
31433975 |
| Submission date |
Jul 01, 2019 |
| Last update date |
Sep 11, 2019 |
| Contact name |
Raquel Buj |
| E-mail(s) |
rbujgomez@pennstatehealth.psu.edu
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| Organization name |
Penn State College of Medicine
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| Department |
Physiology
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| Lab |
Aird
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| Street address |
500 University Drive
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| City |
Hershey |
| State/province |
PA |
| ZIP/Postal code |
17033 |
| Country |
USA |
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| Platforms (1) |
| GPL16791 |
Illumina HiSeq 2500 (Homo sapiens) |
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| Samples (9)
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| Relations |
| BioProject |
PRJNA552100 |
| SRA |
SRP212736 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE133660_DiffExp_RPKM.txt.gz |
2.3 Mb |
(ftp)(http) |
TXT |
| GSE133660_RAW.tar |
1.9 Mb |
(http)(custom) |
TAR (of TXT) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
| Processed data are available on Series record |
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