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Series GSE133844 Query DataSets for GSE133844
Status Public on Mar 17, 2020
Title RNAseq to profile transcriptomes in bone marrow-derived macrophages from TKO (mir-106a~363-/- mir-106b~25-/- mir-17~92flox/flox Lyz2-Cre) and WT(Lyz2-Cre) mice stimulated with LPS for 3 hours.
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary We performed the transcriptomic analysis of RNA-seq of BMDMs generated from WT and TKO mice and stimulated with 10 ng/ml LPS for 3 h to identify gene expression changes.
 
Overall design BMDMs from WT and TKO mice were stimulated with 10 ng/ml LPS for 3h and then were lysed. RNA was extracted, polyA enriched and prepared for sequencing libraries.
 
Contributor(s) Zhang X
Citation(s) 32015501
Submission date Jul 05, 2019
Last update date Mar 18, 2020
Contact name Xiang Zhang
E-mail(s) zinsservictor@gmail.com
Organization name Tsinghua University
Street address Tsinghuayuan Street
City Beijing
ZIP/Postal code 100084
Country China
 
Platforms (1)
GPL23479 BGISEQ-500 (Mus musculus)
Samples (2)
GSM3928265 WT-LPS-3h
GSM3928266 TKO-LPS-3h
Relations
BioProject PRJNA552797
SRA SRP213106

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE133844_WT_LPS_3h-VS-TKO_LPS_3h.txt.gz 2.1 Mb (ftp)(http) TXT
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Raw data are available in SRA
Processed data are available on Series record

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