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Series GSE133854 Query DataSets for GSE133854
Status Public on Oct 08, 2019
Title Single-cell transcriptome analysis of uniparental embryos reveals parent-of-origin effects on human preimplantation development [RNA-seq]
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary To investigate the contribution of parental genomes to early embryogenesis, we systematically profiled the single-cell transcriptomes of human biparental and uniparental embryos from one-cell to morula stages. We observed that uniparental embryos exhibit variable and overall less activation patterns of embryonic genome activation (EGA). Comparative transcriptome analysis identified 807 maternally biased expressed genes (MBGs) and 581 paternally biased expressed genes (PBGs) in preimplantation stages. MBGs became obviously appeared at the four-cell stage and contribute to EGA initiation, whereas PBGs preferentially appeared at the eight-cell stage, and possibly affect embryo compaction and trophectoderm specification. Regulatory network analysis revealed DUX4, EGR2 and DUXA as key transcription factors for MBGs expression as well as ZNF263 and KLF3 for PBGs expression. Furthermore, we revealed that the expression of MBGs and PBGs especially PBGs, probably due to DNA methylation differences between the parental genomes. Together, our results provide a valuable resource to understand parental genome activation and may help to dissect parent-of-origin effect on early human development.
 
Overall design We performed a comprehensively survey of transcriptional activities between biparental and uniparental embryos by single-cell RNA sequencing
BI: biparental, AG: androgenetic, PG: parthenogenetic
 
Contributor(s) Leng L, Lin G
Citation(s) 31588047, 34604214
Submission date Jul 05, 2019
Last update date Oct 06, 2021
Contact name leng lizhi
E-mail(s) lenglizhi.csu@163.com
Organization name Central South University
Street address xiangya road
City Changsha
ZIP/Postal code 410008
Country China
 
Platforms (2)
GPL16791 Illumina HiSeq 2500 (Homo sapiens)
GPL20795 HiSeq X Ten (Homo sapiens)
Samples (296)
GSM3928346 Oocyte_R1
GSM3928347 Oocyte_R2
GSM3928348 Oocyte_R3
This SubSeries is part of SuperSeries:
GSE133856 Single-cell transcriptome analysis of uniparental embryos reveals parent-of-origin effects on human preimplantation development
Relations
BioProject PRJNA552818
SRA SRP213116

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE133854_RAW.tar 48.2 Mb (http)(custom) TAR (of TSV)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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