Granulosa cell expression data from five different time points before and up to 36 hours after induction of ovulation in women receiving fertility treatment
The process of ovulation includes oocyte meiotic maturation, follicle rupture and transformation of the follicle into a corpus luteum. These events are initiated by the midcycle surge of gonadotropins and require the coordinated regulation of thousands of genes. The aim of the study was to monitor the changes in granulosa cell gene expression across five different time points during the first 36 hours of ovulation until follicle rupture, in order to increase our understanding of the events of human ovulation. We conducted a prospective cohort study including women undergoing ovarian stimulation for fertility treatment. Women were treated in a standard antagonist protocol with individually dosed human menopausal gonadotropin (hMG) or recombinant follicle stimulating hormone (rFSH). Ovulation was induced with either recombinant hCG (rhCG) or gonadotropin releasing hormone agonist (GNRHa). The granulosa cells were collected by transvaginal ultrasound-guided follicle puncture of one follicle at two specific time points during ovulation (repeated measurements), and the study covered a total of five time points: before ovulation induction (OI), 12, 17, 32 and 36 hours after OI.
Overall design
Fifty patients were included resulting in 100 samples of granulosa cells (two repeated samples per patient). 17 out of 100 samples were excluded from further analysis because of either insufficient RNA or bad quality of RNA resulting in outliers. The 83 remaining samples represented time points 0h (n=17), 12h (n=7), 17h (n=6), 32h (n=9) and 36h (n=44).
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