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| Status |
Public on Jun 06, 2021 |
| Title |
Single-cell analysis of whole hepatic non-parenchymal cells from normal and CCl4-injured mice |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
This study used 10X Genomics, single-cell RNA-sequencing to examine the heterogeneity of liver non-parenchymal cell types present in the normal and CCl4-mediated fibrotic mice.
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| Overall design |
Two male mice of 8-week old were injected i.p with olive oil and CCl4 independently twice a week for a total 6 weeks. Then, liver non-parenchymal cells were obtained by a two-step collagenase perfusion method. Hepatocytes were elimanated by three times of low-speed centrifugation at 50g for three minutes and were not included in further analysis of this study. Single-cell suspensions were then loaded on a Chromium Single Cell Instrument (10X Genomics) to generate single-cell GEMs. After reverse transcription, libraries were prepared using version 3 Chromium Single-cell 3' Library, Gel Bead & Mutiplex Kit (10X Genomics). Sequencing was performed on illumina HiSeq 3000.
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| Contributor(s) |
Ruan B, Wang L |
| Citation(s) |
34277612, 35114417 |
| Submission date |
Jul 09, 2019 |
| Last update date |
May 05, 2022 |
| Contact name |
Bai Ruan |
| E-mail(s) |
bairuan@fmmu.edu.cn
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| Organization name |
Fourth Military Medical University
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| Street address |
No.169, Changle Road
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| City |
Xi'an |
| State/province |
Shannxi |
| ZIP/Postal code |
710032 |
| Country |
China |
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| Platforms (1) |
| GPL21493 |
Illumina HiSeq 3000 (Mus musculus) |
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| Samples (2) |
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| Relations |
| BioProject |
PRJNA553529 |
| SRA |
SRP213889 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE134037_RAW.tar |
37.9 Mb |
(http)(custom) |
TAR (of XLS) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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