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| Status |
Public on Aug 12, 2020 |
| Title |
A long noncoding RNA landscape in breast cancer reveals a potential role for lncRNA AC009283.1 in proliferation and apoptosis in HER2-enriched molecular subtype |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
We silenced lncRNA AC009283.1 using shRNAs in cell line SKBR3, carried a ~75% silencing compared to thenegative control (NC).
We evaluated the role of lncRNA AC009283.1 on oncogenic phenotypes in the HER2-enriched SKBR3 breast cancer cell line model. The knockdown of lncRNA AC009283.1 was achieved with ~75% of silencing efficiency. A complete transcriptomic analysis after silencing of lncRNA AC009283.1 revealed an impact on the global expression.
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| Overall design |
We analyzed expression profile of knockdown of lncRNA AC009283.1 in SKBR3 cell line using the Affymetrix Human Transcriptome Array 2.0 platform. Array data was processed by Affymetrix Exon Array Computational Tool.
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| Contributor(s) |
Hidalgo-Miranda A, Cedro-Tanda A |
| Citation(s) |
32753692 |
| Submission date |
Jul 15, 2019 |
| Last update date |
Aug 12, 2020 |
| Contact name |
Alberto Cedro-Tanda |
| E-mail(s) |
acedro-tanda@comunidad.unam.mx
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| Organization name |
INMEGEN
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| Lab |
Cancer Genomics
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| Street address |
Periferico sur #4809
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| City |
CDMX |
| State/province |
CMDX |
| ZIP/Postal code |
14610 |
| Country |
Mexico |
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| Platforms (1) |
| GPL17586 |
[HTA-2_0] Affymetrix Human Transcriptome Array 2.0 [transcript (gene) version] |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA554575 |
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