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Series GSE134937 Query DataSets for GSE134937
Status Public on Jun 30, 2020
Title Tandem CAR and single CAR T cells have different fates and therapeutic potency
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary Chimeric antigen receptor T (CAR T) cells targeting CD19 have achieved breakthroughs in the treatment of haematological malignancies, but many clinical studies have also shown that a proportion of patients relapse after remission. In this study, we designed a series of tandem CARs (TanCARs) and found that TanCAR7 T cells retained relatively potent antitumour activity compared with single CAR T cell upon target antigen recognition. It may be associated with stable immunological synapse formation and rapid degranulation. Our transcriptional analysis underscores the potential of scFv domains binding to direct different T cell fates.
 
Overall design In order to assess the different phenotypic and functional patterns of CARs between TanCAR7 and single CAR, we compared the genome-wide transcriptional profiles of TanCAR7, CD19 CAR and CD20 CAR T cell after cocultured with Raji cells for 24 h.
 
Contributor(s) Chuan T
Citation(s) 32556247
Submission date Jul 26, 2019
Last update date Oct 14, 2020
Contact name Tong Chuan
E-mail(s) tc6636@sina.com
Organization name Chinese PLA General Hospital
Department Molecular Biology and Immunology
Street address No. 28 Fuxing Road
City Beijing
State/province Beijing
ZIP/Postal code 100853
Country China
 
Platforms (1)
GPL24676 Illumina NovaSeq 6000 (Homo sapiens)
Samples (6)
GSM3979099 Tancar7_1
GSM3979100 Tancar7_2
GSM3979101 CAR19_1
Relations
BioProject PRJNA556849
SRA SRP216503

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE134937_CRT.6sams.fpkm.txt.gz 413.7 Kb (ftp)(http) TXT
GSE134937_CRT.6sams.nodup_readcount.txt.gz 251.8 Kb (ftp)(http) TXT
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Processed data are available on Series record

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