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Status |
Public on Jun 30, 2020 |
Title |
Tandem CAR and single CAR T cells have different fates and therapeutic potency |
Organism |
Homo sapiens |
Experiment type |
Expression profiling by high throughput sequencing
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Summary |
Chimeric antigen receptor T (CAR T) cells targeting CD19 have achieved breakthroughs in the treatment of haematological malignancies, but many clinical studies have also shown that a proportion of patients relapse after remission. In this study, we designed a series of tandem CARs (TanCARs) and found that TanCAR7 T cells retained relatively potent antitumour activity compared with single CAR T cell upon target antigen recognition. It may be associated with stable immunological synapse formation and rapid degranulation. Our transcriptional analysis underscores the potential of scFv domains binding to direct different T cell fates.
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Overall design |
In order to assess the different phenotypic and functional patterns of CARs between TanCAR7 and single CAR, we compared the genome-wide transcriptional profiles of TanCAR7, CD19 CAR and CD20 CAR T cell after cocultured with Raji cells for 24 h.
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Contributor(s) |
Chuan T |
Citation(s) |
32556247 |
Submission date |
Jul 26, 2019 |
Last update date |
Oct 14, 2020 |
Contact name |
Tong Chuan |
E-mail(s) |
tc6636@sina.com
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Organization name |
Chinese PLA General Hospital
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Department |
Molecular Biology and Immunology
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Street address |
No. 28 Fuxing Road
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City |
Beijing |
State/province |
Beijing |
ZIP/Postal code |
100853 |
Country |
China |
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Platforms (1) |
GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
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Samples (6)
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Relations |
BioProject |
PRJNA556849 |
SRA |
SRP216503 |