|
| Status |
Public on Dec 31, 2019 |
| Title |
Chimeric Antigen Receptor-Induced Bcl11b Suppression Propagates Natural Killer-Like Cell Development |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
The transcription factor B Cell CLL/Lymphoma 11B (BCL11B) is indispensable for T lineage development of lymphoid progenitors. Here we show that chimeric antigen receptor (CAR) expression early in ex vivo generated lymphoid progenitors suppresses Bcl11b, leading to suppression of T cell-associated gene expression and acquisition of natural killer (NK) cell-like properties. These results give important insights into differentiation of murine and human lymphoid progenitors driven by synthetic CAR transgene-expression and inform the potential use of ex vivo generated CARiK cells as a broadly applicable product for targeted immunotherapy.
|
| |
|
| Overall design |
Microarray data analysis of sorted human CAR-engineered lymphoid progenitors (h1928z3: n=3; h19delta: n= 5; h1928z3 + hCD19: n= 3) from OP9-DL1 in vitro cultures on day 18. Human CD34+ HSPCs derived from umbilical cord blood samples were engineered with a CD28-based second-generation human CAR targeting human CD19 (h1928z3) or a non-signaling control CAR (h19delta) and pre-differentiated on OP9-DL1 stromal cells in vitro. For CAR stimulation, irradiated CD19+ Daudi cells were added during the culture (h1928z3_stim).
|
| |
|
| Contributor(s) |
Maluski M, Sauer MG, Geffers R |
| Citation(s) |
31479431 |
| Submission date |
Jul 29, 2019 |
| Last update date |
Apr 10, 2020 |
| Contact name |
Robert Geffers |
| E-mail(s) |
robert.geffers@helmholtz-hzi.de
|
| Phone |
+49 531-6181-3058
|
| Organization name |
HCI - Helmholtz Centre for Infection Research
|
| Department |
Dep. Molecular Bacteriology
|
| Lab |
Genome Analytics
|
| Street address |
Inhoffenstr. 7
|
| City |
Braunschweig |
| ZIP/Postal code |
38124 |
| Country |
Germany |
| |
|
| Platforms (1) |
| GPL23159 |
[Clariom_S_Human] Affymetrix Clariom S Assay, Human (Includes Pico Assay) |
|
| Samples (11)
|
|
| Relations |
| BioProject |
PRJNA557165 |
Less...
More...