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| Status |
Public on Jul 31, 2019 |
| Title |
Transforming growth factor beta 1 induces methylation changes in lung fibroblasts [Illumina] |
| Organism |
Homo sapiens |
| Experiment type |
Methylation profiling by genome tiling array
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| Summary |
Idiopathic pulmonary fibrosis (IPF) is a complex disease of unknown etiology. Environmental factors can affect disease susceptibility via epigenetic effects. Few studies explore global DNA methylation in lung fibroblasts, but none have focused on transforming growth factor beta-1 (TGF-b1) as a potential modifier of the DNA methylome. Here we analyzed changes in methylation and gene transcription in normal and IPF fibroblasts following TGF-b1 treatment.
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| Overall design |
Primary human lung fibroblasts were obtained from normal and IPF lungs. Control, treated for 24 hours and 5 days fibroblasts of both cell lines were selected for DNA extraction, bisulphite conversion, and further hybridization to the Illumina Infinium Human Methylation 450k array.
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| Contributor(s) |
Negreros M, Hagood JS, Espinoza C, Balderas-Martinez YI, Selman M, Pardo A |
| Citation(s) |
31603936 |
| Submission date |
Jul 30, 2019 |
| Last update date |
Oct 30, 2019 |
| Contact name |
Yalbi Itzel Balderas-Martinez |
| E-mail(s) |
yalbibalderas@gmail.com
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| Organization name |
INER
|
| Lab |
Laboratorio de Biología Computacional
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| Street address |
Calzada de Tlalpan 4502, Sección XVI, Belisario Domínguez
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| City |
Ciudad de Mexico |
| State/province |
Distrito Federal |
| ZIP/Postal code |
14080 |
| Country |
Mexico |
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| Platforms (1) |
| GPL13534 |
Illumina HumanMethylation450 BeadChip (HumanMethylation450_15017482) |
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| Samples (12)
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| GSM3985859 |
Lung_fibroblast_normal_control_rep1 [Illumina] |
| GSM3985860 |
Lung_fibroblast_normal_control_rep2 [Illumina] |
| GSM3985861 |
Lung_fibroblast_normal_TGFb_24h_rep1 [Illumina] |
| GSM3985862 |
Lung_fibroblast_normal_TGFb_24h_rep2 [Illumina] |
| GSM3985863 |
Lung_fibroblast_normal_TGFb_5d_rep1 [Illumina] |
| GSM3985864 |
Lung_fibroblast_normal_TGFb_5d_rep2 [Illumina] |
| GSM3985865 |
Lung_fibroblast_IPF_control_rep1 [Illumina] |
| GSM3985866 |
Lung_fibroblast_IPF_control_rep2 [Illumina] |
| GSM3985867 |
Lung_fibroblast_IPF_TGFb_24h_rep1 [Illumina] |
| GSM3985868 |
Lung_fibroblast_IPF_TGFb_24h_rep2 [Illumina] |
| GSM3985869 |
Lung_fibroblast_IPF_TGFb_5d_rep1 [Illumina] |
| GSM3985870 |
Lung_fibroblast_IPF_TGFb_5d_rep2 [Illumina] |
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| This SubSeries is part of SuperSeries: |
| GSE135099 |
Transforming growth factor beta 1 induces methylation changes in lung fibroblasts |
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| Relations |
| BioProject |
PRJNA557428 |
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