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| Status |
Public on Mar 02, 2022 |
| Title |
Rbfox2 is necessary for cardiovascular development |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Purpose: The goal of this study is to investigate the role of Rbfox2, an RNA binding protein in cardiovascular development.
Methods: RNA-Seq splicing analysis of mouse embryonic heart at embryonic day 9.5, isolated from control and genetic deletion of either one or both allels of Rbfox2 using Nkx2-5 Cre.
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| Overall design |
Rbfox2 was deleted from early cardiovascular linease by crossing Rbfox2 floxed mice with Nkx2-5 Cre mice. Effect of Rbfox2 knock-down on alternative splicing and mRNA expression was compared between Control, heterozygous knock-out and homozygous knock-out.
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| Contributor(s) |
Verma SK, Deshmukh V, Thatcher K, Belanger KK, Rhyner AM, Meng S, Bressan M, Martin JF, Cooke JP, Wythe JD, Widen SG, Lincoln J, Kuyumcu-Martinez MN |
| Citation(s) |
35137168 |
| Submission date |
Jul 31, 2019 |
| Last update date |
Mar 02, 2022 |
| Contact name |
Muge N Kuyumcu-Martinez |
| E-mail(s) |
nmmartin@utmb.edu
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| Phone |
409-7723228
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| Organization name |
University of Texas Medical Branch
|
| Department |
Biochemistry and Molecular Biology
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| Lab |
Kuyumcu-Martinez lab
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| Street address |
301 University Blvd
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| City |
Galveston |
| State/province |
TX |
| ZIP/Postal code |
77555 |
| Country |
USA |
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| Platforms (1) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA557594 |
| SRA |
SRP216887 |
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