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| Status |
Public on Feb 11, 2020 |
| Title |
Transcriptome analysis of CHI3L1 knockdown NP cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Intervertebral disc degeneration is the main cause of low back pain and the mechanism of which is far from fully revealed. Although multiple factors are related to the intervertebral disc degeneration, inflammation and matrix metabolism dysregulation are the two key factors that play an important role in degeneration. Here, we found that inflammation-related factor CHI3L1 is highly regulated in the nucleus pulposus during degeneration in both RNA and protein level. Immunohistochemical analysis show that the expression of CHI3L1 are NP tissue specific, and increased significantly in the degenerated nucleus pulposus cells. The mechanism of CHI3L1 is thus studied in this experiment.
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| Overall design |
MRNA profiles were done using primary culture cells of normal human intervertebral disc nucleus pulposus (NP) tissue. Each sample were treated with IL-1b, TNF-a, IL-1b+CHI3L1 (100ug/ml) or TNF-a+CHI3L1 (100ug/ml) for 48h.
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| Contributor(s) |
Xu C, Wang R, Yuan W |
| Citation(s) |
30301907 |
| Submission date |
Aug 01, 2019 |
| Last update date |
Feb 11, 2020 |
| Contact name |
Chen Xu |
| E-mail(s) |
chenxu8836@hotmail.com
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| Phone |
+86-13774294166
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| Organization name |
The Second Military Medical University
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| Street address |
No, 800, Rd. Xiangyin
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| City |
Shanghai |
| ZIP/Postal code |
200433 |
| Country |
China |
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| Platforms (1) |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA557839 |
| SRA |
SRP217041 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE135219_all.counts.txt.gz |
492.9 Kb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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