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Status |
Public on Dec 12, 2020 |
Title |
FAM83H-AS1 silencing in MCF7 breast cancer cells |
Organism |
Homo sapiens |
Experiment type |
Expression profiling by array
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Summary |
We silenced FAM83H-AS1 shRNAs in cell line MCF7 carried a ~75% silencing compared to thenegative control (NC). We evaluated the role of FAM83H-AS1 on oncogenic phenotypes in the MCF7 breast cancer cell line model. The knockdown of FAM83H-AS1 was achieved with ~75% of silencing efficiency. A complete transcriptomic analysis after silencing of FAM83H-AS1 revealed an impact on the global expression.
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Overall design |
We analyzed expression profile of knockdown of FAM83H-AS1 in MCF7 cell line using the Affymetrix Human Transcriptome Array 2.0 platform. Array data was processed by Affymetrix Exon Array Computational Tool.
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Contributor(s) |
Hidalgo-Miranda A, Ríos-Romero M |
Citation missing |
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Submission date |
Aug 06, 2019 |
Last update date |
Dec 14, 2020 |
Contact name |
Alberto Cedro-Tanda |
E-mail(s) |
acedro-tanda@comunidad.unam.mx
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Organization name |
INMEGEN
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Lab |
Cancer Genomics
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Street address |
Periferico sur #4809
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City |
CDMX |
State/province |
CMDX |
ZIP/Postal code |
14610 |
Country |
Mexico |
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Platforms (1) |
GPL17586 |
[HTA-2_0] Affymetrix Human Transcriptome Array 2.0 [transcript (gene) version] |
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Samples (6)
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Relations |
BioProject |
PRJNA558978 |