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Series GSE135466 Query DataSets for GSE135466
Status Public on Dec 12, 2020
Title FAM83H-AS1 silencing in MCF7 breast cancer cells
Organism Homo sapiens
Experiment type Expression profiling by array
Summary We silenced FAM83H-AS1 shRNAs in cell line MCF7 carried a ~75% silencing compared to thenegative control (NC).
We evaluated the role of FAM83H-AS1 on oncogenic phenotypes in the MCF7 breast cancer cell line model. The knockdown of FAM83H-AS1 was achieved with ~75% of silencing efficiency. A complete transcriptomic analysis after silencing of FAM83H-AS1 revealed an impact on the global expression.
 
Overall design We analyzed expression profile of knockdown of FAM83H-AS1 in MCF7 cell line using the Affymetrix Human Transcriptome Array 2.0 platform. Array data was processed by Affymetrix Exon Array Computational Tool.
 
Contributor(s) Hidalgo-Miranda A, Ríos-Romero M
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Submission date Aug 06, 2019
Last update date Dec 14, 2020
Contact name Alberto Cedro-Tanda
E-mail(s) acedro-tanda@comunidad.unam.mx
Organization name INMEGEN
Lab Cancer Genomics
Street address Periferico sur #4809
City CDMX
State/province CMDX
ZIP/Postal code 14610
Country Mexico
 
Platforms (1)
GPL17586 [HTA-2_0] Affymetrix Human Transcriptome Array 2.0 [transcript (gene) version]
Samples (6)
GSM4010843 NC_1: Negative Control shRNA_1
GSM4010844 NC_2: Negative Control shRNA_2
GSM4010845 NC_3: Negative Control shRNA_3
Relations
BioProject PRJNA558978

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE135466_RAW.tar 155.7 Mb (http)(custom) TAR (of CEL, CHP)
Processed data included within Sample table
Processed data provided as supplementary file

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