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| Status |
Public on Aug 10, 2019 |
| Title |
Robust hepatitis E virus infection and transcriptional response in human hepatocytes |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
In this study, we established a protocol based on the HEV genotype 3 p6 (Kernow C-1) and the human hepatoma cell lines HepG2 and HepG2/C3A with different media conditions to produce HEV cell culture-derived particles (HEVcc) with viral titers between 10e5 to 10e6 FFU/mL. Viral titers could be further enhanced by an HEV variant harboring a mutation in the RNA-dependent RNA polymerase. These HEVcc particles were infectious in primary human hepatocytes. RNA sequencing studies of HEV infected primary human hepatocytes demonstrated a temporally structured transcriptional defense response. In conclusion, this robust cell culture model of HEV infection provides a powerful tool for studying viral-host interactions that should facilitate the discovery of antiviral drugs and vaccines for this important zoonotic pathogen.
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| |
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| Overall design |
Primary human hepatocytes (PHH) were inoculated with cell culture-derived HEV particles and changes in transcriptional profiles were assessed using RNAseq.
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| |
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| Contributor(s) |
Todt D, Friesland M, Steinmann E |
| Citation(s) |
31896581 |
| Submission date |
Aug 09, 2019 |
| Last update date |
Jan 06, 2020 |
| Contact name |
Daniel Todt |
| E-mail(s) |
daniel.todt@rub.de
|
| Phone |
+492343222463
|
| Organization name |
Ruhr University Bochum
|
| Department |
Molecular & Medical Virology
|
| Street address |
Universitätsstr. 150
|
| City |
Bochum |
| ZIP/Postal code |
44801 |
| Country |
Germany |
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| Platforms (1) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
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| Samples (24)
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| Relations |
| BioProject |
PRJNA559467 |
| SRA |
SRP217907 |
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