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| Status |
Public on Nov 04, 2019 |
| Title |
Amino acid licensing of regulatory T cell function requires signaling via small G proteins Rag and Rheb |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
Amino acids license Treg cell function by priming and sustaining TCR induced mTORC1 activity and Rag and Rheb GTPases as central regulators of mTORC1 activation in effector Treg (eTreg) cells
RNA was purified from CD25+ Treg cells isolated from Cd4CreRragafl/flRragabfl/fl or Cd4CreRhebfl/flRhebl1–/– mice or their littermate controls.
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| Overall design |
In total 20 samples were analyzed: WT (3 Unsimulated and 3 Stimulated); Rheb1/2 KO (3 Unsimulated and 3 Stimulated) and additional 8 samples of Tregs (2 WT and 2 RagAB KO unstimulated) and (2 WT and 2 RagAB KO Tregs stimulated for 8 hours).
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| Contributor(s) |
Shi H, Chapman N, Dhungana Y, Chi H |
| Citation(s) |
31668641 |
| Submission date |
Aug 12, 2019 |
| Last update date |
Nov 06, 2019 |
| Contact name |
Hongbo Chi |
| E-mail(s) |
hongbo.chi@stjude.org
|
| Organization name |
St Jude Children's Research Hospital
|
| Department |
Immunology
|
| Street address |
262 Danny Thomas Place
|
| City |
Memphis |
| State/province |
TN |
| ZIP/Postal code |
38105 |
| Country |
USA |
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| Platforms (1) |
| GPL23038 |
[Clariom_S_Mouse] Affymetrix Clariom S Assay, Mouse (Includes Pico Assay) |
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| Samples (20)
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| Relations |
| BioProject |
PRJNA559891 |
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