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Status |
Public on Jan 28, 2009 |
Title |
Use of spermatozoal total RNA profiles to study gene-environment interactions in human germ cells |
Organism |
Homo sapiens |
Experiment type |
Expression profiling by array
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Summary |
Paternal exposure to genotoxic compounds is thought to contribute to diseases in their offspring. Therefore, mRNA profiles from sperm in ejaculates of cigarette smokers (N=4) were compared with non-smokers (N=4). Smoking behaviour was verified by assessing cotinine levels in seminal plasma. Gene-expression analysis was subsequently performed using microarray technology (Agilent human 4x44k) to investigate the use of gene-expression profiling as biomarker of gene-environment interactions in human testis
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Overall design |
The study investigated differential gene expression in spermatozoal RNA from 4 smokers as compared to 4 non-smokers. Two conditions: smoking or non-smoking. One individual per array, hybridized against a common reference sample.
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Contributor(s) |
Linschooten JO, van Schooten FJ, Baumgartner A, Cemeli E, van Delft J, Anderson D, Godschalk RW |
Citation(s) |
19409198 |
Submission date |
Nov 13, 2008 |
Last update date |
Jan 23, 2019 |
Contact name |
Joost Linschooten |
E-mail(s) |
Joost.Linschooten@GRAT.unimaas.nl
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Phone |
+31 (0)43 388 1088
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URL |
http://www.grat.nl
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Organization name |
Maastricht University
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Department |
Health Risk Analysis & Toxicology
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Street address |
Universiteitssingel 50
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City |
Maastricht |
ZIP/Postal code |
PO Box 616, 6200 MD |
Country |
Netherlands |
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Platforms (1) |
GPL6480 |
Agilent-014850 Whole Human Genome Microarray 4x44K G4112F (Probe Name version) |
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Samples (8)
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Relations |
BioProject |
PRJNA110217 |