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| Status |
Public on Oct 01, 2019 |
| Title |
Genome-wide view of the impact of Spt5-Pol II inhibitors (SPIs) on transcription [GRO-Seq] |
| Organism |
Homo sapiens |
| Experiment type |
Other
|
| Summary |
We identified the first Spt5-Pol II inhibitors (SPIs). SPIs faithfully reproduced Spt5 knockdown effects on proximal-promoter-pausing, NF-κB activation and the expanded-repeat huntingtin gene in neuronal cells. Using SPIs we identified Spt5 target genes that responded with profoundly diverse kinetics and a novel regulatory element of proximal-promoter-pausing. To obtain a genome-wide view of the impact of SPIs on transcription, we performed Global run-on sequencing (GRO-seq) using nuclei of HeLa cells treated with either DMSO (control) or SPI-21.
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| Overall design |
Nuclei were isolated from HeLa cells pre-treated with either DMSO (control) or SPI-21 (50μM) for 1.5 hours
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| Contributor(s) |
Dikstein R, Bahat A, Leshkowitz D |
| Citation(s) |
31564557 |
| Submission date |
Aug 19, 2019 |
| Last update date |
Oct 01, 2019 |
| Contact name |
Dena Leshkowitz |
| E-mail(s) |
dena.leshkowitz@weizmann.ac.il
|
| Organization name |
Weizmann Institute of Science
|
| Department |
Bioinformatics Unit, Life Sciences Core Facilities
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| Street address |
P.O.B. 26
|
| City |
Rehovot |
| ZIP/Postal code |
76100 |
| Country |
Israel |
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| Platforms (1) |
| GPL16791 |
Illumina HiSeq 2500 (Homo sapiens) |
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| Samples (3) |
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| This SubSeries is part of SuperSeries: |
| GSE136026 |
Targeting Spt5-Pol II small-molecule inhibitors uncouple distinct activities and reveal additional regulatory roles |
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| Relations |
| BioProject |
PRJNA560966 |
| SRA |
SRP218860 |
