|
| Status |
Public on Oct 01, 2019 |
| Title |
Genome-wide view of the impact of Spt5-Pol II inhibitors (SPIs) on transcription [4sU-seq] |
| Organism |
Homo sapiens |
| Experiment type |
Other
|
| Summary |
We identified the first Spt5-Pol II inhibitors (SPIs). SPIs faithfully reproduced Spt5 knockdown effects on proximal-promoter-pausing, NF-κB activation and the expanded-repeat huntingtin gene in neuronal cells. Using SPIs we identified Spt5 target genes that responded with profoundly diverse kinetics and a novel regulatory element of proximal-promoter-pausing. To validate that the effects of SPIs are at the transcriptional level, cellular RNA was metabolically labeled with 4-thio-uridine (4sU) for 2 hours in the presence of DMSO or SPI-21 in the presence or absence of TNFα. Newly synthesized labeled RNA was then purified and subjected to RNA-seq.
|
| |
|
| Overall design |
Cellular RNA was metabolically labeled with 4-thio-uridine (4sU) for 2 hours in the presence of DMSO or SPI-21, in the presence or absence of TNFα.
|
| |
|
| Contributor(s) |
Dikstein R, Bahat A, Leshkowitz D |
| Citation(s) |
31564557 |
| Submission date |
Aug 19, 2019 |
| Last update date |
Oct 01, 2019 |
| Contact name |
Dena Leshkowitz |
| E-mail(s) |
dena.leshkowitz@weizmann.ac.il
|
| Organization name |
Weizmann Institute of Science
|
| Department |
Bioinformatics Unit, Life Sciences Core Facilities
|
| Street address |
P.O.B. 26
|
| City |
Rehovot |
| ZIP/Postal code |
76100 |
| Country |
Israel |
| |
|
| Platforms (1) |
| GPL16791 |
Illumina HiSeq 2500 (Homo sapiens) |
|
| Samples (8)
|
|
| This SubSeries is part of SuperSeries: |
| GSE136026 |
Targeting Spt5-Pol II small-molecule inhibitors uncouple distinct activities and reveal additional regulatory roles |
|
| Relations |
| BioProject |
PRJNA560967 |
| SRA |
SRP218861 |
Less...
More...