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| Status |
Public on Jan 13, 2021 |
| Title |
High-throughput sequencing of extracellular vesicles from human dermal papilla cells |
| Organism |
Homo sapiens |
| Experiment type |
Non-coding RNA profiling by high throughput sequencing
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| Summary |
The use of dermal papilla cells for hair follicle (HF) regeneration is long accepted much attention. However, cultured dermal papilla cells tend to lose the hair-inducible capability during passaging, which restricts its application. Increasing evidences indicate that dermal papilla cells exert their regulatory function of HF growth mainly through their unique paracrine properties, opening up a way to exosome therapies.This study aimed to explore the effects of exosomes from high and low-passaged human scalp follicle dermal papilla cells (DP-Exos) on hair follicle stem cells (HFSCs) activation and hair growth, and to investigate the underline mechanism. DP-Exos were isolated by ultracentrifugation and cultured with human scalp follicles and HFSCs. The hair elongation and cell proliferation was assessed. Quantitative real-time PCR (qRT-PCR) and Western-blot were performed to detect the expression levels of a class of miRNAs and proteins which have positive roles in regulating hair growth and HFSCs proliferation. High throughput miRNA sequencing of miRNAs in high (P8) and low-passaged (P3) DP-Exos was performed, and the utmost miRNA and its target gene was identified via bioinformatics analysis.
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| Overall design |
DP-Exos were isolated by ultracentrifugation from dermal papilla cells. High throughput miRNA sequencing of miRNAs in high (P8) and low-passaged (P3) DP-Exos was performed.
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| Contributor(s) |
Chen Y, Huang J |
| Citation(s) |
33425897 |
| Submission date |
Aug 23, 2019 |
| Last update date |
Jan 13, 2021 |
| Contact name |
Yuxin Chen |
| E-mail(s) |
doctorjf@126.com
|
| Organization name |
Nanfang Hospital of Southern Medical University
|
| Street address |
Nanfang Dadao Street, Baiyun district
|
| City |
Guangzhou |
| ZIP/Postal code |
510515 |
| Country |
China |
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| Platforms (1) |
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| Samples (6)
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| GSM4043014 |
Dermal papilla-derived exosomes, P3-Exos1 |
| GSM4043015 |
Dermal papilla-derived exosomes, P3-Exos2 |
| GSM4043016 |
Dermal papilla-derived exosomes, P3-Exos3 |
| GSM4043017 |
Dermal papilla-derived exosomes, P8-Exos1 |
| GSM4043018 |
Dermal papilla-derived exosomes, P8-Exos2 |
| GSM4043019 |
Dermal papilla-derived exosomes, P8-Exos3 |
|
| Relations |
| BioProject |
PRJNA561717 |
| SRA |
SRP219159 |
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