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| Status |
Public on Feb 04, 2020 |
| Title |
Role of engrailed 2 (EN2) in esophageal squamous cell carcinoma |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
A number of homeobox genes have been found to be implicated in the development of various cancers. Here, we investigated the role of engrailed 2 (EN2), a member of the homeobox gene superfamily, in esophageal squamous cell carcinoma
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| Overall design |
RNA profiling was performed using gene expression microarray. A lentivirus system was used to establish a stable cell line expressing exogenous EN2. The malignant phenotype was analyzed by means of proliferation, clonogenicity, wound-healing, and invasion assays. The CRISPR/Cas9 system was adopted to deplete the endogenous EN2. The ShRNA-mediated method was used to knock down the expression of SPARC. Separately, the structure–function relationship was determined by the site-directed mutagenesis method.
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| Contributor(s) |
Cao Y, Wang X, Tang L, Li Y, Song X, Liu X, Li M, Chen F, Wan H |
| Citation(s) |
32117645 |
| Submission date |
Aug 26, 2019 |
| Last update date |
Mar 09, 2020 |
| Contact name |
xu liu |
| E-mail(s) |
liu-xu@hotmail.com
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| Organization name |
The Affiliated Hospital of Southwest Medical University
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| Street address |
25th Taiping Street
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| City |
Luzhou |
| ZIP/Postal code |
646000 |
| Country |
China |
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| Platforms (2) |
| GPL23126 |
[Clariom_D_Human] Affymetrix Human Clariom D Assay [transcript (gene) version] |
| GPL23159 |
[Clariom_S_Human] Affymetrix Clariom S Assay, Human (Includes Pico Assay) |
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| Samples (10)
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| Relations |
| BioProject |
PRJNA562267 |
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