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Status |
Public on Dec 31, 2020 |
Title |
RNA-seq analysis after depletion of ALDH1A2 by enhancer blocking in Jurkat cells |
Organism |
Homo sapiens |
Experiment type |
Expression profiling by high throughput sequencing
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Summary |
RNA-seq analysis was performed in a T-cell acute lymphoblastic leukemia cell line (Jurkat) to analyze gene expression changes after ALDH1A2 depletion.
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Overall design |
The inducible single-guide RNA mediated dCas9-KRAB system was used to transcriptionally deplete ALDH1A2 gene expression. Jurkat cells expressing the catalytic dead Cas9 fused with KRAB peptide was first established. The cells were then infected with lentivirus encoding an inducible sgRNA targeting the regulatory element of ALDH1A2 gene. Total RNA was harvested 72hrs upon induction of sgRNA and applied for RNA-seq analysis.
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Contributor(s) |
Zhang C, Tan TK, Sanda T |
Citation missing |
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Submission date |
Aug 27, 2019 |
Last update date |
Dec 31, 2020 |
Contact name |
Tze King Tan |
Organization name |
National University of Singapore
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Department |
Cancer Science Institute
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Lab |
Takaomi Sanda Lab
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Street address |
14 Medical Drive
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City |
Singapore |
ZIP/Postal code |
117599 |
Country |
Singapore |
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Platforms (1) |
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Samples (4)
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Relations |
BioProject |
PRJNA562523 |
SRA |
SRP219388 |