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Series GSE13646 Query DataSets for GSE13646
Status Public on May 18, 2011
Title Global effect of JTV or JTV-Alt overexpression in HeLa cells
Organism Homo sapiens
Experiment type Expression profiling by array
Summary JTV is not only a structural component of multi-aminoacyl-tRNA synthetase complex, but also a regulator of FBP that in turn controls c-Myc expression. In order to identify JTV target genes other than FBP and c-Myc, JTV or JTV-Alt (alternatively spliced form of JTV, enriched in the nucleus) protein was over-expressed in HeLa cells and global gene expression changes were monitored using cDNA expression microarray.
 
Overall design JTV or JTV-Alt was expressed in HeLa cells by transient transfection as protein fusion with GFP. After harvesting, only GFP-positive cells were isolated by flow cytometry, and used for RNA extraction. Each JTV or JTV-Alt sample was analyzed by co-hybridizing with control GFP-only samples. Each of the two forms of JTV hybridization was repeated as biological replicate.
 
Citation(s) 21285945
Submission date Nov 18, 2008
Last update date Mar 20, 2012
Contact name David Levens
E-mail(s) levens@helix.nih.gov
Phone 301-496-2176
Fax 301-402-0043
Organization name NIH
Department NCI/CCR
Lab LP
Street address 10 Center Drive
City Bethesda
State/province MD
ZIP/Postal code 20892
Country USA
 
Platforms (1)
GPL3779 NCI/ATC Hs-OperonV3
Samples (4)
GSM343494 GFP-JTV Transfection - repeat 1 - mAdbID:75970
GSM343495 GFP-JTV-Alt Transfection - repeat 1 - mAdbID:75971
GSM343496 GFP-JTV Transfection - repeat 2 - mAdbID:77132
Relations
BioProject PRJNA110197

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE13646_RAW.tar 15.9 Mb (http)(custom) TAR (of GPR)
Processed data included within Sample table
Raw data provided as supplementary file
Raw data included within Sample table

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