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| Status |
Public on Aug 01, 2021 |
| Title |
C. elegans PPM-2 regulates small RNA function by stabilizing Argonaute proteins |
| Organism |
Caenorhabditis elegans |
| Experiment type |
Non-coding RNA profiling by high throughput sequencing
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| Summary |
Argonaute proteins associate with small non-coding RNAs such as microRNAs, piRNAs and siRNAs to regulate gene expression required for cell proliferation and differentiation. Thse Argonaute proteins are the major components of effector complexes of non-coding RNA pathways. In C. elegans, we identified PPM-2, a Mg2+/Mn2+-dependent serine/threonine phosphatase, as a new regulator of the small RNA pathways in C. elegans. We demonstrate that PPM-2 is involved in the regulation of the let-7 microRNA family but also in the regulation of nuclear RNAi as well as it contributes to the expression of piRNAs. The loss of ppm-2 affects the expression level of Type 1 piRNAs and the stability of ALG-1, PRG-1 and NRDE-3 Argonaute proteins by sending them to the proteasomal degradation pathway. Altogether, our findings suggest that PPM-2 is regulating the function of small non-coding RNA pathways by preventing Argonaute proteins to be degraded via the proteasomal pathway.
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| Overall design |
Develop a novel protocol to clone small RNA and mRNA
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| Contributor(s) |
Fressigné L, Houle F, Bossé GD, Li L, Proulx-Landry G, Gu W, Simard MJ |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Aug 27, 2019 |
| Last update date |
Aug 02, 2021 |
| Contact name |
Weifeng Gu |
| Organization name |
University of California at Riverside
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| Department |
Molecular, Cell and Systems Biology
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| Lab |
Weifeng Gu
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| Street address |
900 University Ave
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| City |
Riverside |
| State/province |
California |
| ZIP/Postal code |
92521 |
| Country |
USA |
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| Platforms (1) |
| GPL13657 |
Illumina HiSeq 2000 (Caenorhabditis elegans) |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA562557 |
| SRA |
SRP219914 |
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