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| Status |
Public on Nov 19, 2008 |
| Title |
Deep surveying of alternative splicing complexity in the human transcriptome by high-throughput sequencing |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
We carried out the first analysis of alternative splicing complexity in human tissues using mRNA-Seq data. New splice junctions were detected in 20% of multiexon genes, many of which are tissue specific. By combining mRNA-Seq and EST-cDNA sequence data, we estimate that transcripts from 95% of multiexon genes undergo alternative splicing and that there are 100,000 intermediate- to high-abundance alternative splicing events in major human tissues. From a comparison with quantitative alternative splicing microarray profiling data, we also show that mRNA-Seq data provide reliable measurements for exon inclusion levels.
Keywords: mRNA expression
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| Overall design |
32-nucleotide sequence reads from six human tissues including brain, cerebral cortex, heart, liver, lung and skeletal muscle.
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| Contributor(s) |
Pan Q, Shai O, Lee LJ, Frey BJ, Blencowe BJ |
| Citation(s) |
18978789 |
| Submission date |
Nov 18, 2008 |
| Last update date |
May 15, 2019 |
| Contact name |
Weijun Gao |
| Organization name |
University of Toronto
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| Street address |
10 King's College Rd.
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| City |
Toronto |
| ZIP/Postal code |
M5S 3G4 |
| Country |
Canada |
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| Platforms (1) |
| GPL9052 |
Illumina Genome Analyzer (Homo sapiens) |
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| Samples (6)
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| Relations |
| SRA |
SRP000626 |
| BioProject |
PRJNA110203 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE13652_RAW.tar |
2.7 Gb |
(http)(custom) |
TAR (of TXT) |
SRA Run Selector |
| Processed data provided as supplementary file |
| Raw data are available in SRA |
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