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| Status |
Public on Sep 18, 2019 |
| Title |
Understanding the stromal composition of fat-associated lymphoid clusters of the omentum using single-cell transcriptomics |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
We profile the transcriptome of over 4000 murine single cells from the stromal non-endothelial fraction of the omentum. We uncover that the omentum is composed of 2 populations of fibroblasts which differentially express Ccl11 and Ccl19 and three populations of mesothelial cells. One of these mesothelial populations were distinguised by the expression of inflammatory genes and the other one by the expression of anti-viral genes. This study unveil the complexcity of the stromal niche in the omentum and revealed the adaptation of the mesothelium to FALCs function.
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| Overall design |
Single-cell transcriptomic data are presented from the pooled omentum of 3 unchallenged mice. Cell were sorted as CD45-Ter119-CD41-CD31-Gp38+
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| Contributor(s) |
Smith P, Portman JR, Henderson B, Dobie R, Henderson N, Benezech C |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Aug 29, 2019 |
| Last update date |
Jan 29, 2020 |
| Contact name |
Cecile Benezech |
| E-mail(s) |
cbenezec@ed.ac.uk
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| Organization name |
University of Edinburgh
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| Department |
Centre for Cardiovascular Sciences
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| Street address |
47 Little france Crescent
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| City |
Edinburgh |
| ZIP/Postal code |
EH16 4TJ |
| Country |
United Kingdom |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (1) |
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| Relations |
| BioProject |
PRJNA562904 |
| SRA |
SRP219839 |
