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Series GSE13670 Query DataSets for GSE13670
Status Public on Apr 23, 2009
Title Gene expression data from S. aureus-exposed macrophages
Organism Homo sapiens
Experiment type Expression profiling by array
Summary It is becoming increasingly apparent that Staphylococcus aureus are able to survive engulfment by macrophages, and that the intracellular environment of these cells, which is essential to innate host defenses against invading microorganisms, may in fact provide a refuge for staphylococcal survival and dissemination. Based on this, we postulated that S. aureus might induce cytoprotective mechanisms by changing gene expression profiles inside macrophages similar to obligate intracellular pathogens, such as Mycobacterium tuberculosis.
In order to examine the effect of S. aureus on the macrophage transcriptome, we performed microarray expression analysis on human monocyte-derived macrophages treated with S. aureus.

Keywords: time course
 
Overall design Human monocyte-derived macrophages (hMDMs) were separated from fractions of peripheral blood mononuclear cells (PBMCs) obtained from the blood of healthy donors. Control and S. aureus-exposed macrophages were incubated at 37C for 8, 24, or 48 hours
 
Contributor(s) Koziel J, Maciag-Gudowska A, Mikolajczyk T, Bzowksa M, Whitney AR, Sturdevant DE, Shaw LN, DeLeo FR, Potempa J
Citation(s) 19381294
Submission date Nov 19, 2008
Last update date Jul 15, 2019
Contact name Addie Whitney
E-mail(s) awhitney@niaid.nih.gov
Organization name NIH/NIAID/RML
Department LHBP
Lab DeLeo
Street address 903 S 4th St
City Hamilton
State/province MT
ZIP/Postal code 59840
Country USA
 
Platforms (1)
GPL570 [HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array
Samples (30)
GSM343802 hMDM_control_8hr_1
GSM343803 hMDM_SA_8hr_1
GSM343804 hMDM_control_24hr_1
Relations
BioProject PRJNA110411

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE13670_RAW.tar 152.7 Mb (http)(custom) TAR (of CEL, CHP)
Raw data provided as supplementary file
Processed data included within Sample table
Processed data provided as supplementary file

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