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| Status |
Public on Sep 23, 2019 |
| Title |
RNA-seq Analysis of mRNA profiles in Purβ-overexpressing and Purβ-knocking down hepatocytes |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Purpose: The goal of this study is to investigate how Purβ regulates hepatic glucose production in primary hepatocytes.
Methods: mRNA profiles of Purβ-overexpressing, and Purβ-knocking down hepatocytes were generated by deep sequencing using an Illumina NovaSeq 6000 platform. Paired-end clean reads were aligned to the mouse reference genome(Ensemble_GRCm38.96) with TopHat (version 2.0.12), and the aligned reads were used to quantify mRNA expression by using HTSeq-count (version 0.6.1).
Conclusion: Our study represents the first detailed analysis of mRNA profiles in Purβ-overexpressing, and Purβ-knocking down hepatocytes, generated by RNA-seq technology. Our results identified Adcy6 was a target of Purβ. RNA-seq analysis showed that Purβ overexpression increased Adcy6 expression, whereas Purβ knockdown decreased Adcy6 expression.
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| Overall design |
mRNA profiles of Purβ-overexpressing, and Purβ-knocking down hepatocytes were generated by deep sequencing using an Illumina NovaSeq 6000 platform.
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| Contributor(s) |
Chen Z, Jia L |
| Citation |
Linna Jia, Yunfeng Jiang, Xinzhi Li, and Zheng Chen. Purβ promotes hepatic glucose production by increasing Adcy6 transcription. January 2020, Volume 31, Pages 85-97. doi:10.1016/j.molmet.2019.11.008
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| Submission date |
Sep 02, 2019 |
| Last update date |
Dec 23, 2019 |
| Contact name |
Zheng Chen |
| E-mail(s) |
chenzheng@hit.edu.cn
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| Phone |
+8645186402029
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| Organization name |
Harbin Institute of Technology
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| Street address |
92 Xidazhijie Harbin Institute of Technology
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| City |
Harbin |
| State/province |
Heilongjiang |
| ZIP/Postal code |
150001 |
| Country |
China |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA563345 |
| SRA |
SRP220361 |
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