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| Status |
Public on Sep 30, 2019 |
| Title |
BAT Hi-C maps global chromatin interactions in an efficient and economical way |
| Organism |
Mus musculus |
| Experiment type |
Other
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| Summary |
We describe the Bridge linker-Alul-Tn5 Hi-C (BAT Hi-C) method, which is a simple and efficient method for delineating chromatin conformational features of mouse embryonic stem (mES) cells and uncover DNA loops.
This protocol combines Alul fragmentation and biotinylated linker-mediated proximity ligation to obtain kilobase (kb) resolution with a marked increase in the amount of unique read pairs.
The protocol also includes chromatin isolation to reduce background noise and Tn5 tagmentation to cut down on preparation time.
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| Overall design |
BAT Hi-C data in Untreated mES cells. Reference: The SRA Study accession (SRP218781) and BioProject accession (PRJNA561038)
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| Contributor(s) |
Huang J, Jiang Y |
| Citation(s) |
31442560 |
| BioProject |
PRJNA561038 |
| Submission date |
Sep 03, 2019 |
| Last update date |
Dec 30, 2019 |
| Contact name |
Xiong Ji |
| E-mail(s) |
xiongji@pku.edu.cn
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| Organization name |
Peking University
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| Department |
School of Life Sciences
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| Lab |
Jilab
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| Street address |
Haidian District, Beijing Summer Palace Road No. 5
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| City |
Beijing |
| ZIP/Postal code |
100871 |
| Country |
China |
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| Platforms (1) |
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| Samples (2) |
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| Relations |
| SRA |
SRP218781 |
