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| Status |
Public on Oct 18, 2019 |
| Title |
Genome-wide mapping of oxidative DNA damage via engineering of 8-oxoguanine DNA glycosylase |
| Organism |
Mus musculus |
| Experiment type |
Other
|
| Summary |
7,8-dihydro-8-oxoguanine (OG) is one of the major DNA oxidative damages, which has been concerned by researchers for its potential of mutagenesis and epigenetic regulation. Genome-wide mapping of oxidative DNA damage is of great value for understanding the biological impact of these modifications. Here we present a novel appoach to profile guanine oxidation in the mouse genome. Through structure-guided mutagenesis, we've successufully engineered 8-oxoguanine DNA glycosylase into a useful tool for OG detection. By enrichment of glycosylase-DNA complex, thousands of OG peaks were identified in the mouse genome.
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| Overall design |
Enrichment procedure was perform on mouse embryonic fibroblasts (MEFs) with two biological replicates. Genomic samples without any treatment were denoted as input; samples enriched by engineered glycosylase were denoted as enrich; samples pretreated with Fpg to remove oxidative damages before enrichment were denoted as control.
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| Contributor(s) |
Fang Y, Zou P |
| Citation(s) |
31618020 |
| Submission date |
Sep 04, 2019 |
| Last update date |
Feb 20, 2020 |
| Contact name |
Yuxin Fang |
| E-mail(s) |
fangyx201@pku.edu.cn
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| Phone |
15122297861
|
| Organization name |
Peking University
|
| Street address |
Yiheyuan Road No.5
|
| City |
Beijing |
| State/province |
Beijing |
| ZIP/Postal code |
100871 |
| Country |
China |
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| Platforms (1) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA563884 |
| SRA |
SRP220329 |
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