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| Status |
Public on Nov 20, 2009 |
| Title |
Effect of TCPOBOP and PCN in combination with high-cholesterol diet on genes involved in cholesterol homeostasis |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
TCPOBOP (1,4-bis[2-(3,5-dichloropyridyloxy)]benzene) and PCN (pregnenolone 16α-carbonitrile) are inducers of drug metabolism through activation of nuclear receptors CAR (constitutive androstane receptor) and PXR (pregnane X receptor), respectively. Mouse experiment was designed to study the effect of CAR and PXR activation on cholesterol homeostasis genes and other genes, which are present on the Steroltalk v2 microarray. Treatments were combined with standard and high-cholesterol diet to observe the interference of high liver cholesterol on nuclear receptor transcription regulation. All experiments were done within the European sixth Framework program “Steroltalk” (www.steroltalk.net). Results form these experiments give new knowledge about involvement of ‘xenosensors’ CAR and PXR in regulation of endogenous liver metabolism.
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| Overall design |
Animals were injected i.p. 3mg/kg TCPOBOP, 40 mg/kg PCN or vehicle (corn oil) in combination of one week of standard or 1% cholesterol diet prior tretament. After 24h they were sacrificed and total RNA was isolated from the livers. Each sample was hybridized with a reference sample to Steroltalk v2 microarrays.
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| Contributor(s) |
Režen T, Rozman D |
| Citation(s) |
19691840 |
| Submission date |
Nov 20, 2008 |
| Last update date |
Mar 20, 2012 |
| Contact name |
Peter Juvan |
| Phone |
+386 1 543 7595
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| Organization name |
Faculty of Medicine, University of Ljubljana
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| Department |
Institute of Biochemistry
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| Lab |
Center for Functional Genomics and Bio-Chips
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| Street address |
Zaloska 4
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| City |
Ljubljana |
| ZIP/Postal code |
SI-1000 |
| Country |
Slovenia |
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| Platforms (1) |
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| Samples (42)
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| This SubSeries is part of SuperSeries: |
| GSE13721 |
Effect of TCPOBOP, PCN, rosuvastatin, and atorvastatinin on cholesterol homeostasis |
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| Relations |
| BioProject |
PRJNA114573 |
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