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Series GSE136937 Query DataSets for GSE136937
Status Public on Nov 03, 2021
Title Changes in gut microbiota induced by oral pathobiont deteriorate the pathology of non-alcoholic fatty liver disease
Organism Mus musculus
Experiment type Expression profiling by array
Summary Periodontitis increases the risk of non-alcoholic fatty liver disease (NAFLD). However, the precise mechanisms are unclear. Here, gut dysbiosis induced by orally administered Porphyromonas gingivalis, a representative periodontopathic bacterium, was implicated in the deterioration of NAFLD pathology. C57BL/6 mice were administered with the vehicle, P. gingivalis or Prevotella intermedia, with weaker periodontal pathogenicity, followed by feeding on a choline-deficient, high fat diet (CDAHFD60). CDAHFD60 feeding induced hepatic steatosis, and combined bacterial administration further aggravated NAFLD pathology with increased fibrosis. Liver gene expression analyses revealed that genes involved in the NAFLD pathology were perturbed with distinctive expression profiles induced by different bacteria. These differences may be due to quantitative and qualitative differences in the influx of gut bacterial products because the serum endotoxin level, gut microbiota composition, and serum metabolite profile caused by ingested P. intermedia and P. gingivalis were different. These findings provide insights into mechanisms linking periodontitis and NAFLD.
 
Overall design Effect of oral administration of P. intermedia and P. gingivalis on the liver gene expression profile was mesured in high fat diet-fed mice. Mice were divided into 4 groups ( regular chow, no bacteria (n=3), high fat diet, sham (n=4), high fat diet, P. intermdia (n=4), high fat diet, P. gingivalis (n=5)). Bacteria were administered 2 times aweek for 3 weeks nd high fat diet was fed for 2 weeks.
 
Contributor(s) Yamazaki K, Miura N, Okuda S, Yamazaki K, Kato T
Citation(s) 34707622
Submission date Sep 05, 2019
Last update date Nov 04, 2021
Contact name Kazuhisa Yamazaki
E-mail(s) kaz@dent.niigata-u.ac.jp
Organization name Niigata University
Street address 2-110-1 Gakkocho-dori, Chu-o-ku
City Niigata
ZIP/Postal code 951-8514
Country Japan
 
Platforms (1)
GPL21163 Agilent-074809 SurePrint G3 Mouse GE v2 8x60K Microarray [Probe Name version]
Samples (16)
GSM4062010 Regular chow_no bacteria_rep1
GSM4062011 Regular chow_no bacteria_rep2
GSM4062012 Regular chow_no bacteria_rep3
Relations
BioProject PRJNA564083

Download family Format
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE136937_RAW.tar 61.4 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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