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| Status |
Public on Sep 06, 2019 |
| Title |
Single cell RNAseq analysis of Cxcl12-tdTomato+ bone marrow stromal cells after marrow ablation, Day 7 |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
A comparative single cell transcriptional analysis of murine CXCL12-abundant reticular stromal cells isolated from ablated and control bone marrow
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| Overall design |
Lineage-marked Cxcl12-iCreER+ cells were FACS-isolated individually from ablated and contralateral control femurs of Cxcl12-iCreER; R26R-tdTomato mice at 7 days after bone marrow ablation, with tamoxifen injection at 7 days before ablation. 6,500~14,000 cells were loaded onto 10X Genomic Chromium Single Cell Expression Solution version 2 or 3 platform. cDNA was sequenced by Illumina HiSeq 4000 or NovaSeq 6000.
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| |
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| Contributor(s) |
Ono N |
| Citation(s) |
31949165 |
| Submission date |
Sep 05, 2019 |
| Last update date |
Jan 27, 2020 |
| Contact name |
Noriaki Ono |
| E-mail(s) |
noriaki.ono@uth.tmc.edu
|
| Phone |
7134860539
|
| Organization name |
University of Texas Health Science Center at Houston
|
| Department |
School of Dentistry
|
| Lab |
Ono lab
|
| Street address |
1941 East Rd, Rm 5322
|
| City |
Houston |
| State/province |
Texas |
| ZIP/Postal code |
77054 |
| Country |
USA |
| |
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| Platforms (2) |
| GPL21103 |
Illumina HiSeq 4000 (Mus musculus) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA564146 |
| SRA |
SRP220536 |
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