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| Status |
Public on Aug 31, 2020 |
| Title |
GM-CSF-transduced proliferating myeloid cells represent cell cycle related gene signature |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
We previously established a method to generate myeloid lineage cells with proliferation capacity from induced pluripotent stem cells (iPSCs) (Zhang R. Cancer Immunol Res. 3: 668, 2015). In this study, we generated the GM-CSF-producing cells by genetic engineering of mouse iPSC-derived proliferation myeloid cells (iPSC-pMCs). Gene expression profiles revealed GM-CSF-transduced iPSC-pMCs (GM-pMCs) generated from C57BL/6 mice and 129Sv mice shared 180 up-regulated genes and 236 down-regulated genes compared with their corresponding iPSC-pMCs. Gene ontology analysis showed that the up-regulated gene signature shared by C57BL/6 GM-pMCs and 129Sv GM-pMCs was enriched for transcripts associate with cell cycle, cell cycle process, mitotic cell cycle and regulation of mitotic cell cycle.
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| Overall design |
Gene expression profiles of C57BL/6 iPSC-pMCs, GM-CSF-transuduced C57BL/6 iPSC-pMCs, GM-CSF-treated C57BL/6 iPSC-pMCs, 129Sv iPSC-pMCs, GM-CSF-transuduced 129Sv iPSC-pMCs, GM-CSF-treated 129Sv iPSC-pMCs
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| |
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| Contributor(s) |
Zhang R, Mahima H |
| Citation(s) |
33457097 |
| Submission date |
Sep 06, 2019 |
| Last update date |
Jan 19, 2021 |
| Contact name |
Minaho Kawamura |
| Organization name |
Rhelixa.Inc
|
| Street address |
BLICK GATE Suidobashi F2, Chiyodaku-kandamisakicho
|
| City |
Tokyo |
| ZIP/Postal code |
101-0061 |
| Country |
Japan |
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| Platforms (1) |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA564231 |
| SRA |
SRP220635 |
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