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| Status |
Public on Jun 24, 2020 |
| Title |
RNA Sequencing of C elegans control and mutant worms after 24 hour P.aeruginosa infection |
| Organism |
Caenorhabditis elegans |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Purpose: After infection there was significant decrease in the lifespan of mutant animals . To decipher the mechanism responsible and the changes in the gene expression of mutant worms
Methods: Control and mutant worms were exposed at young adult stage to P.aeruginosa PA14 infection for 24 hours. Total RNA was extracted and RNA sequencing was done following standard protocols
Results: Detailed analysis of the transcriptomic data show that immune genes were significantly upregulated which have role in lifespan
Conclusions: This study showed the role of pathogenic infection in the life span modulation
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| |
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| Overall design |
C. elegans RNA seq of young adults of control and mutant worms after 24 hour P.aeruginosa PA14 infection in triplicate, using Illumina platform
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| |
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| Contributor(s) |
Kaur S, Aballay A |
| Citation(s) |
32433971 |
| Submission date |
Sep 09, 2019 |
| Last update date |
Jun 24, 2020 |
| Contact name |
Supender Kaur |
| E-mail(s) |
supender@ohsu.edu
|
| Organization name |
Oregon health and science university
|
| Department |
Molecular Microbiology and Immuno
|
| Lab |
6351 RICHARD JONES HALL
|
| Street address |
3181 SW Sam Jackson Park Road L220
|
| City |
Portland |
| State/province |
Oregon |
| ZIP/Postal code |
97239 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL13657 |
Illumina HiSeq 2000 (Caenorhabditis elegans) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA564575 |
| SRA |
SRP221060 |
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