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Series GSE13743 Query DataSets for GSE13743
Status Public on May 21, 2010
Title Comparison of naïve, postmitotic (CD44-lo/CD62L-hi) and effector (CD44-hi/CD62L-lo) CD8+ Tcells during BMT in GVHD mice
Organism Mus musculus
Experiment type Expression profiling by array
Summary Naïve CD44-lo/CD62L-hi/CD8+ T cells from C3H.SW mice were compared to CD44-hi/CD82L-lo/CD8+ effector memory T cells and CD44-lo/CD62L-hi/CD8+ postmitotic T cells, using 3 biological replicates of each type of sample. The later two cells types were highly purified at day 14 after transplantation from GVHD B6/SJL mice receiving donor C3H.SW mouse-derived naive CD44-lo/CD62L-hi/CD8+ T cells and T cell-depleted bone marrow. Recipient mice had first been lethally irradiated at a dose of 10Gy in two fractions. This is a MHC-identical minor histocompatibility antigen-mismatched mouse GVHD model of human allogeneic hematopoietic stem cell transplantation. Naive T cell samples were from pools of 2 mice each, while effector memory and postmitotic T cell samples were purified from pools of T cells from 4 mice each. After RNA extraction and cleanup, biotin labeled cRNA was prepared from 600 ng total RNA, using two rounds of in vitro transcription, and hybridized to Affymetrix Mouse Genome 430A 2.0 arrays using standard techniques.

Keywords: Cell type comparison
 
Overall design 9 samples were analyzed on 9 Affymetrix microarrays to assay mRNA levels. There were 3 biological replicates of each of 3 different cell types.
 
Contributor(s) Kato K, Cui S, Mineishi S, Kuick R, Wang J, Bacon E, Huang J, Klein P, Hexner E, Jiang J, Reddy P, Ferrara JL, Emerson SG, Zhang Y
Citation(s) 20116439
Submission date Nov 25, 2008
Last update date May 04, 2018
Contact name Rork Kuick
E-mail(s) rork@umich.edu
Phone 734-936-9241
Organization name University of Michigan
Street address University of Michigan, SPH II, 1415 Washington Heights, Room M2533
City Ann Arbor
State/province MI
ZIP/Postal code 48109-2029
Country USA
 
Platforms (1)
GPL8321 [Mouse430A_2] Affymetrix Mouse Genome 430A 2.0 Array
Samples (9)
GSM345404 Naïve CD44-lo/CD62L-hi/CD8+ T cells, biol rep 1
GSM345405 Naïve CD44-lo/CD62L-hi/CD8+ T cells, biol rep 2
GSM345406 Naïve CD44-lo/CD62L-hi/CD8+ T cells, biol rep 3
Relations
BioProject PRJNA109393

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE13743_RAW.tar 17.5 Mb (http)(custom) TAR (of CEL)
GSE13743_Zhang_arrays_logs.xls 15.9 Mb (ftp)(http) XLS
GSE13743_Zhang_supplement_readme.txt 2.9 Kb (ftp)(http) TXT
Processed data included within Sample table

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