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GEO help: Mouse over screen elements for information. |
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Status |
Public on May 21, 2010 |
Title |
Comparison of naïve, postmitotic (CD44-lo/CD62L-hi) and effector (CD44-hi/CD62L-lo) CD8+ Tcells during BMT in GVHD mice |
Organism |
Mus musculus |
Experiment type |
Expression profiling by array
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Summary |
Naïve CD44-lo/CD62L-hi/CD8+ T cells from C3H.SW mice were compared to CD44-hi/CD82L-lo/CD8+ effector memory T cells and CD44-lo/CD62L-hi/CD8+ postmitotic T cells, using 3 biological replicates of each type of sample. The later two cells types were highly purified at day 14 after transplantation from GVHD B6/SJL mice receiving donor C3H.SW mouse-derived naive CD44-lo/CD62L-hi/CD8+ T cells and T cell-depleted bone marrow. Recipient mice had first been lethally irradiated at a dose of 10Gy in two fractions. This is a MHC-identical minor histocompatibility antigen-mismatched mouse GVHD model of human allogeneic hematopoietic stem cell transplantation. Naive T cell samples were from pools of 2 mice each, while effector memory and postmitotic T cell samples were purified from pools of T cells from 4 mice each. After RNA extraction and cleanup, biotin labeled cRNA was prepared from 600 ng total RNA, using two rounds of in vitro transcription, and hybridized to Affymetrix Mouse Genome 430A 2.0 arrays using standard techniques.
Keywords: Cell type comparison
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Overall design |
9 samples were analyzed on 9 Affymetrix microarrays to assay mRNA levels. There were 3 biological replicates of each of 3 different cell types.
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Contributor(s) |
Kato K, Cui S, Mineishi S, Kuick R, Wang J, Bacon E, Huang J, Klein P, Hexner E, Jiang J, Reddy P, Ferrara JL, Emerson SG, Zhang Y |
Citation(s) |
20116439 |
Submission date |
Nov 25, 2008 |
Last update date |
May 04, 2018 |
Contact name |
Rork Kuick |
E-mail(s) |
rork@umich.edu
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Phone |
734-936-9241
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Organization name |
University of Michigan
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Street address |
University of Michigan, SPH II, 1415 Washington Heights, Room M2533
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City |
Ann Arbor |
State/province |
MI |
ZIP/Postal code |
48109-2029 |
Country |
USA |
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Platforms (1) |
GPL8321 |
[Mouse430A_2] Affymetrix Mouse Genome 430A 2.0 Array |
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Samples (9)
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GSM345404 |
Naïve CD44-lo/CD62L-hi/CD8+ T cells, biol rep 1 |
GSM345405 |
Naïve CD44-lo/CD62L-hi/CD8+ T cells, biol rep 2 |
GSM345406 |
Naïve CD44-lo/CD62L-hi/CD8+ T cells, biol rep 3 |
GSM345407 |
CD44-hi/CD62L-lo/CD8+ effector memory T cells, biol rep 1 |
GSM345408 |
CD44-hi/CD62L-lo/CD8+ effector memory T cells, biol rep 2 |
GSM345409 |
CD44-hi/CD62L-lo/CD8+ effector memory T cells, biol rep 3 |
GSM345410 |
CD44-lo/CD62L-hi/CD8+ postmitotic T cells, biol rep 1 |
GSM345411 |
CD44-lo/CD62L-hi/CD8+ postmitotic T cells, biol rep 2 |
GSM345412 |
CD44-lo/CD62L-hi/CD8+ postmitotic T cells, biol rep 3 |
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Relations |
BioProject |
PRJNA109393 |
Supplementary file |
Size |
Download |
File type/resource |
GSE13743_RAW.tar |
17.5 Mb |
(http)(custom) |
TAR (of CEL) |
GSE13743_Zhang_arrays_logs.xls |
15.9 Mb |
(ftp)(http) |
XLS |
GSE13743_Zhang_supplement_readme.txt |
2.9 Kb |
(ftp)(http) |
TXT |
Processed data included within Sample table |
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