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| Status |
Public on Sep 25, 2019 |
| Title |
Glycine Cleavage System Determines the Fate of Pluripotent Stem Cells via the Regulation of Senescence and Epigenetic Modifications |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
To investigate whether embryonic stem cells exhibit a metabolic state different from that of somatic cells, we performed RNA-seq in mouse embryonic fibroblasts (MEFs), induced pluripotent stem cells (iPSCs) and mouse embryonic stem cells (mESCs). To further investigate the effect of Gldc in mESCs, we performed RNA-seq in V6.5 cells with Gldc knockdown.
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| Overall design |
RNA-seq for MEF,IPS and V6.5 cells was performed to analyze the gene expression of these lines. mouse ES cell were transfected with Gldc shRNA or non-targeting control shRNA followed by RNA extraction and sequenced on HiSeq X Ten (Mus musculus).
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| Contributor(s) |
Tian S, Feng J, Shen S, Zhang H, Zhong X, Gao P |
| Citation(s) |
31562192 |
| Submission date |
Sep 13, 2019 |
| Last update date |
Oct 08, 2019 |
| Contact name |
junru feng |
| E-mail(s) |
jrfeng@mail.ustc.edu.cn
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| Phone |
+860051 63607033
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| Organization name |
USTC
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| Street address |
huangshan road #443
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| City |
Hefei |
| ZIP/Postal code |
230026 |
| Country |
China |
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| Platforms (1) |
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| Samples (5)
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| Relations |
| BioProject |
PRJNA565440 |
| SRA |
SRP221611 |
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