|
| Status |
Public on Dec 31, 2019 |
| Title |
Role of Cyp2b in diet-induced nonalcoholic steatohepatitis (NASH) |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
To investigate the role of hepatic CYP2B in diet-induced nonalcoholic steatohepatitis (NASH), a Cyp2b triple knockout mouse lacking Cyp2b9, Cyp2b10, and Cyp2b13 was developed using CRISPER/Cas9. Wildtype (WT) and Cyp2b-null mice were fed a normal diet (ND) or a choline-deficient, L-amino acid-defined high-fat diet (CDAHFD), containing 0.1% methionine and 62% fat for 8 weeks. RNA was extracted from the livers of female and male mice from all treatment groups and used for RNA seqencing. RNAseq data demonstrated that a lack of Cyp2b was protective in female but more harmful in male mice. Hepatic gene expression revealed a higher number of phase I-III xenobiotic metabolism and inflammatory response genes were down-regulated in CDAHFD-fed WT female and Cyp2b-null male mice.
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| Overall design |
Use RNA-sequencing to investigate the role of Cyp2b in diet-induced NASH on a transcriptomic level, by comparing the livers of WT and Cyp2b-null mice fed a CDAHFD for 8 weeks using Illumina technology.
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| Contributor(s) |
Heintz MM, Noorai RE, Baldwin WS |
| Citation(s) |
32155178 |
| NIH grant(s) |
| Grant ID |
Grant title |
Affiliation |
Name |
| R15 ES017321 |
Does the inhibition of key xenobiotic-metabolizing CYPs cause obesity? |
CLEMSON UNIVERSITY |
William S Baldwin |
| P20 GM109094 |
Clemson University Genomics Institute (CUGI) |
CLEMSON UNIVERSITY |
Christopher Alan Saski |
|
| Submission date |
Sep 14, 2019 |
| Last update date |
Mar 31, 2020 |
| Contact name |
William S Baldwin |
| E-mail(s) |
baldwin@clemson.edu
|
| Phone |
915-491-7366
|
| Organization name |
Clemson University
|
| Department |
Biological Sciences
|
| Lab |
Dr. William Baldwin
|
| Street address |
230 Parkway Dr
|
| City |
Clemson |
| State/province |
South Carolina |
| ZIP/Postal code |
29634 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
|
| Samples (32)
|
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| Relations |
| BioProject |
PRJNA565506 |
| SRA |
SRP221637 |
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