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Series GSE13749 Query DataSets for GSE13749
Status Public on Nov 24, 2009
Title Genome-wide characterization of the transcriptional program of Myc-dependent transformation, ChIP-chip
Organism Homo sapiens
Experiment type Genome binding/occupancy profiling by array
Summary We profiled Myc binding in a normal breast cell-line (MCF10A) under basal conditions after ectopic expression of Myc. We showed that ectopic Myc expression increases tumour formation in vivo and in vitro. We then profiled genome-wide Myc binding using Agilent promoter arrays in MCF10A cells expressing wild-type and ectopic Myc. We show that Myc binds to a greater number of spots in wild-type than in Myc cells, but that some targets are unique to each condition.

Keywords: ChIP-chip
 
Overall design We compared ectopic Myc to wild-type cells with n=10 per condition
 
Contributor(s) Wasylishen AR, Boutros PC, Ponzielli R, Jurisica I, Penn LZ
Citation missing Has this study been published? Please login to update or notify GEO.
Submission date Nov 26, 2008
Last update date Dec 06, 2012
Contact name Paul C Boutros
E-mail(s) Paul.Boutros@utoronto.ca
Organization name Ontario Institute for Cancer Research
Street address 101 College Street, Suite 800
City Toronto
State/province Ontario
ZIP/Postal code M5G 0A3
Country Canada
 
Platforms (2)
GPL4124 Agilent-014706 Human Promoter ChIP-on-Chip Set 244K, Microarray 1 of 2 G4489A (Feature Number version)
GPL4125 Agilent-014707 Human Promoter ChIP-on-Chip Set 244K, Microarray 2 of 2 G4489A (Feature Number version)
Samples (40)
GSM346071 MCF10A-MycChIPchip-ectopicGFP-rep1_chipA
GSM346072 MCF10A-MycChIPchip-ectopicGFP-rep2_chipA
GSM346073 MCF10A-MycChIPchip-ectopicGFP-rep3_chipA
This SubSeries is part of SuperSeries:
GSE14264 Genome-wide characterization of the transcriptional program of Myc-dependent transformation
Relations
BioProject PRJNA114639

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE13749_RAW.tar 6.3 Gb (http)(custom) TAR (of TIFF, TXT)
Processed data included within Sample table
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