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| Status |
Public on Jan 27, 2021 |
| Title |
Transcriptomic profiling of wild-type C2C12 cells and C2C12 cells with Myod1 gene knockout by RNA-sequencing |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
C2C12 is a myoblast cell line usually used for study of muscle differentiation. Myod1 is a pro-differentiation factor for myogenesis. We found that CRISPR/Cas9-mediated Myod1 gene knockout in C2C12 cells led to the loss of myoblast identity and gain of neural properties.
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| Overall design |
Myod1 gene was knocked out with CRISPR/Cas9. Wild type C2C12 cells (C2C12(WT)) and gene knockout cells (C2C12(Myod1-/-)) showed different phenotypes when cultured in serum-free medium. Each three samples of C2C12(WT) and C2C12(Myod1-/-) were collected and subjected to RNA-sequencing.
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| Contributor(s) |
Cao Y, Zhang M |
| Citation(s) |
33468253 |
| Submission date |
Sep 16, 2019 |
| Last update date |
Jan 27, 2021 |
| Contact name |
Ying Cao |
| E-mail(s) |
caoying@nju.edu.cn
|
| Organization name |
Nanjing University
|
| Street address |
12 Xuefu Rd., Pukou High-Tech Zone
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| City |
Nanjing |
| ZIP/Postal code |
210061 |
| Country |
China |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA565753 |
| SRA |
SRP221751 |
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