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| Status |
Public on Sep 19, 2020 |
| Title |
Novel Immune Modulators Enhance Caenorhabditis elegans Resistance to Multiple Pathogens |
| Organism |
Caenorhabditis elegans |
| Experiment type |
Expression profiling by array
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| Summary |
Traditional treatments for bacterial infection have focused upon directly inhibiting growth of the pathogen. However, an equally important determinant of infection outcome is the host defense response. We previously performed a high-throughput chemical screen to identify small molecules that rescued the nematode Caenorhabditis elegans from infection by Pseudomonas aeruginosa. Over 20 of the hits stimulated host defense gene expression. During in-depth studies of five such molecules using microarray analysis, bioinformatic clustering, and RNAi knockdown of candidate gene targets, we identified PMK-1/p38 MAPK and SKN-1/Nrf2 as two key pathways modulated by these hits. Interestingly, the molecules studied did not depend on a single pathway for ameliorating P. aeruginosa pathogenesis in liquid-based assay, but did rely on the PMK-1/p38 MAPK pathway during a colonization-based infection assay on agar. A subset of these molecules was also protective against Enterococcus faecalis and Staphylococcus aureus. In general, the compounds showed little toxicity against mammalian cells or worms, consistent with their identification in a phenotypic, high-content screen. These molecules possess significant potential for use as tools to study innate immune processes
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| Overall design |
There are 18 samples total that comprise three biological replicates of N2 young adult animals exposed to LK32, LK34, LK35, LK38, LK56, or DMSO control
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| Contributor(s) |
Kirienko NV |
| Citation(s) |
33408224 |
| Submission date |
Sep 16, 2019 |
| Last update date |
Feb 01, 2021 |
| Contact name |
Natalia V Kirienko |
| E-mail(s) |
kirienko@rice.edu
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| Organization name |
Rice University
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| Department |
BioSciences
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| Lab |
Kirienko
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| Street address |
6100 Main St. MS140
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| City |
Houston |
| State/province |
TX |
| ZIP/Postal code |
77005 |
| Country |
USA |
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| Platforms (1) |
| GPL200 |
[Celegans] Affymetrix C. elegans Genome Array |
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| Samples (18)
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| Relations |
| BioProject |
PRJNA565765 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE137516_RAW.tar |
35.2 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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