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| Status |
Public on Jul 10, 2020 |
| Title |
Dysregulation of microRNA expression in diabetic skin |
| Platform organism |
synthetic construct |
| Sample organism |
Mus musculus |
| Experiment type |
Non-coding RNA profiling by array
|
| Summary |
Clinical skin manifestations are common in diabetes; however, molecular mechanisms underlying such defects are largely unknown. Several findings indicate a role for microRNA (miRNA) in skin homeostasis. We investigated whether miRNA expression is altered in the skin of a Type 1 mouse model of diabetes. For this purpose, miRNA profiling by microarray analysis was performed on RNA extracted from the skin of diabetic mice and non-diabetic controls. >400 different miRNA species were identified, differential expression-analysis revealed miRNA dysregulation in diabetic skin. Interestingly, among the 30 most significantly modulated genes, 27 were downregulated and 3 were upregulated in diabetic mice. Pathway analysis using Tarbase showed an enrichment of signature-miRNA target genes in pathways with roles in skin homeostasis, such as TGF-β and Wnt.
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| Overall design |
Total RNAs from the back skin of streptozotocin-induced diabetic mice (a model of type I diabetes) and non-diabetic controls were extracted and miRNA profiling by microarray analysis was performed.
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| Web link |
https://0-www-sciencedirect-com.brum.beds.ac.uk/science/article/pii/S092318112030147X?via%3Dihub
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| Contributor(s) |
Baldini E, Testa E, De Domenico E, Cianfarani F, Voellenkle C, Martelli F, Ulisse S, Odorisio T |
| Citation(s) |
32402513 |
| Submission date |
Sep 17, 2019 |
| Last update date |
Jul 12, 2020 |
| Contact name |
Fabio Martelli |
| E-mail(s) |
fabio.martelli@grupposandonato.it
|
| Phone |
+390252774533
|
| Organization name |
IRCCS-Policlinico San Donato
|
| Lab |
Molecular Cardiology
|
| Street address |
via morandi 30
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| City |
Milan |
| ZIP/Postal code |
20097 |
| Country |
Italy |
| |
|
| Platforms (1) |
| GPL17107 |
Exiqon miRCURY LNA™ microRNA Array - hsa, mmu & rno |
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| Samples (8)
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| GSM4082977 |
Diabetic.1_versus_pool of non-diabetic |
| GSM4082978 |
Diabetic.2_versus_pool of non-diabetic |
| GSM4082979 |
Diabetic.2_versus_pool of non-diabetic_dye swap |
| GSM4082980 |
Diabetic.4_versus_pool of non-diabetic |
| GSM4082981 |
Diabetic.6_versus_pool of non-diabetic |
| GSM4082982 |
Diabetic.7_versus_pool of non-diabetic |
| GSM4082983 |
Diabetic.7_versus_pool of non-diabetic_dye swap |
| GSM4082984 |
Diabetic.9_versus_pool of non-diabetic |
|
| Relations |
| BioProject |
PRJNA565989 |
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