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| Status |
Public on Sep 30, 2020 |
| Title |
Abnormal neocortex arealization and Sotos-like syndrome-associated behavior in Setd2 mutant mice |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
Epigenetic regulation of gene expression through histone modifications like methylation of various lysine residues are essential for embryonic development. Here we removed SETD2, a methyltransferase for histone 3 lysine 36 trimethylation (H3K36me3), in the developing dorsal forebrain in mice and show it is required for proper area patterning (arealization) of the neocortex and the formation of thalamo-cortico-thalamic circuits by maintaining the expression of clustered protocadherin (Pcdh) genes in an H3K36me3 methyltransferase-dependent manner. Moreover, the Setd2 mutant mice exhibit defects in social interaction, motor endurance and spatial memory, reminiscent of patients with the Sotos-like syndrome bearing SETD2 mutations.
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| Overall design |
H3K36me3 ChIP-seq profiles of E13.5 control and Emx1-Cre;Setd2 fl/fl mice cortex were generated by deep sequencing, in two replicates, using Illumina Hi-seq X Ten.
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| Contributor(s) |
Xu L, Zheng Y, Li X, Wang A, Huo D, Li Q, Wang S, Luo Z, Liu Y, Xu F, Wu X, Wu M, Zhou Y |
| Citation(s) |
33523829 |
| Submission date |
Sep 18, 2019 |
| Last update date |
Feb 08, 2021 |
| Contact name |
Lichao Xu |
| E-mail(s) |
lichao.xu@whu.edu.cn
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| Organization name |
Wuhan University
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| Street address |
luojiashan
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| City |
Wuhan |
| ZIP/Postal code |
430000 |
| Country |
China |
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| Platforms (1) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA566085 |
| SRA |
SRP222234 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE137622_RAW.tar |
408.2 Mb |
(http)(custom) |
TAR (of BW) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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