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Series GSE137653 Query DataSets for GSE137653
Status Public on Dec 16, 2019
Title polyA+ RNA sequencing on FACS sorted alveolar macrophages (CD45+SiglecF+CD11c+) from air and cigarette-smoke exposed wild type and miR-155 KO mice
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Chronic obstructive pulmonary disease (COPD) is a highly prevalent respiratory disease characterized by airflow limitation and chronic inflammation. MiR-155 is described as an ancient regulator of the immune system. Our objective was to establish a role for miR-155 in cigarette smoke (CS)-induced inflammation and COPD. We demonstrate increased miR-155 expression by RT-qPCR in lung tissue of smokers without airflow limitation and patients with COPD compared to never smokers and in lung tissue and alveolar macrophages of CS-exposed mice compared to air-exposed mice. In addition, we exposed wild type and miR-155 deficient mice to CS and show an attenuated inflammatory profile in the latter. Alveolar macrophages were sorted by FACS from the different experimental groups and their gene expression profile was analyzed by RNA sequencing. This analysis revealed increased expression of miR-155 targets (including the NF-κB inhibitor rassf6) and an attenuation of the CS-induced increase in inflammation-related genes in miR-155 deficient mice. Finally, intranasal instillation of a specific miR-155 inhibitor significantly attenuated the CS-induced pulmonary inflammation in mice. In conclusion, we highlight a role for miR-155 in CS-induced inflammation and the pathogenesis of COPD, implicating miR-155 as a new therapeutic target in COPD.
Overall design poly-A+ RNA sequencing was performed on 5 mice per group (4 groups: air_wild-type, air_miR-155-KO, CS_wild-type, CS_miR-155-KO)
Contributor(s) De Smet EG, Avila Cobos F, Bracke KR
Citation(s) 31819170
Submission date Sep 18, 2019
Last update date Dec 16, 2019
Contact name Ken R Bracke
Organization name Ghent University
Department Department of Respiratory Medicine
Street address Corneel Heymanslaan 10
City Ghent
ZIP/Postal code 9000
Country Belgium
Platforms (1)
GPL19057 Illumina NextSeq 500 (Mus musculus)
Samples (20)
GSM4083803 Alveolar macrophages, WTAIR_Ia3
GSM4083805 Alveolar macrophages, WTAIR_Ia4
GSM4083807 Alveolar macrophages, WTAIR_Ib1
BioProject PRJNA566153
SRA SRP222324

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Supplementary file Size Download File type/resource
GSE137653_logCPM_limma-voom.txt.gz 1.9 Mb (ftp)(http) TXT
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Raw data are available in SRA
Processed data are available on Series record

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