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| Status |
Public on Dec 15, 2019 |
| Title |
CD48-dependent protective autophagy in conventional human T cells promotes restimulation-induced cell death resistance |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Transcriptome profiling and functional analyses on expanding Tcons revealed that FOXP3 enhances expression of the SLAM family receptor CD48, which in turn sustains basal autophagy and suppresses pro-apoptotic p53 signaling. Our findings suggest that FOXP3 governs a distinct transcriptional program in early-stage effector Tcons that maintains RICD resistance via CD48-dependent protective autophagy and p53 suppression.
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| Overall design |
Purified human CD4 T cells were electroporated with siRNAs against CD48 or negative-control medum GC duplex siRNA and differential gene expression analysis was performed using mRNA sequencing.
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| Contributor(s) |
Dalgard CL, Snow AL |
| Citation missing |
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| Submission date |
Sep 24, 2019 |
| Last update date |
Dec 15, 2019 |
| Contact name |
Clifton Lee Dalgard |
| E-mail(s) |
cdalgard@usuhs.edu
|
| Organization name |
Uniformed Services University
|
| Department |
Anatomy Physiology & Genetics
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| Lab |
Dalgard
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| Street address |
4301 Jones Bridge Road B2038
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| City |
Bethesda |
| State/province |
Maryland |
| ZIP/Postal code |
20814 |
| Country |
USA |
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| Platforms (1) |
| GPL21290 |
Illumina HiSeq 3000 (Homo sapiens) |
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| Samples (10)
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| Relations |
| BioProject |
PRJNA573899 |
| SRA |
SRP223086 |
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