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| Status |
Public on Sep 26, 2019 |
| Title |
Next Generation Sequencing Facilitates Quantitative Analysis of TFH2 cell Transcriptomes |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Emergence of IgE antibodies that bind allergens with high affinity is highly correlated with the severity of anaphylaxis. However, the underlying molecular mechanisms by which high-affinity IgE is generated remain poorly understood. We discovered that IL-13-producing T follicular helper (TFH) cells, which we designated as TFH2 cells, are generated from conventional TH2 cells by antigen sensitization. TFH2 cells have a unique cytokine profile (IL-4+IL-5-IL-13+IL-21+) and co-express the TFH and TH2 master transcriptional regulators, BCL-6 and GATA3.
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| |
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| Overall design |
Naïve CD4T, TH2, or TFH cells of mice were sorted and analyzed the transcriptome using Illumina HiSeq1500.
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| Contributor(s) |
Kubo M |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Sep 25, 2019 |
| Last update date |
Sep 28, 2019 |
| Contact name |
Kosuke Miyauchi |
| E-mail(s) |
kosuke.miyauchi@riken.jp
|
| Organization name |
RIKEN Center for Integrative Medical Sciences
|
| Street address |
1-7-22 Suehiro-sho
|
| City |
Yokohama |
| State/province |
Kanagawa |
| ZIP/Postal code |
230-0045 |
| Country |
Japan |
| |
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| Platforms (1) |
| GPL18480 |
Illumina HiSeq 1500 (Mus musculus) |
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| Samples (30)
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| Relations |
| BioProject |
PRJNA574058 |
| SRA |
SRP223238 |
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